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《Acta Sericologica Sinica》 2005-03
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Composition Analysis of Protein From Posterior Silkgland of Silkworm Bombyx mori Between Larvae of 4~(th) Molting Stage and the 5~(th) Instar Stage

WU Weicheng~1,2 ZHONG Boxiong~(1*) MENG Zhiqi~2 CHEN Jin’e~1,2YAN Xinpei~1 YE Jian~1 SHEN Feiying~1(~1 Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029,China;~2 Laboratory of Molecular Biology of Insect, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China)  
Changes of protein forms from posterior silkgland of silkworm Bombyx mori between larvae of 4~th molting and the 5~th instar stage were analyzed by using 2D-PAGE and image analysis technique for inquiring into regulation mechnism of relative gene expression during synthesis and secretion proteins in silkgrand cell. The 425 protein spots were detected in posterior silkgland of 4~th molting larva. New special protein spots could be detected along with growth of the silkworm, which revaled that the protein forms in posterior silkgland cell is different at different stage. The special protein spots appeared during prophase and metaphase of 5~th instar stage may associated with the synthesis and secretion of fibroin protein, while the special protein spots appeared in anaphase of 5~th instar stage may associated with the changes of configuration of silkgland tissue and silkgland cell apoptosis.
【Fund】: 浙江省科技厅项目(编号2004C32048)。
【CateGory Index】: S881.2
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