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《Acta Sericologica Sinica》 2005-03
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Genetic Diversity Among Philosamia cynthia ricini Strains Revealed by ISSRs

HOU Chengxiang~1 LI Muwang~1,2,3 MIAO Xuexia~2 XU Anying~1SUN Pingjiang~1 ZHANG Yuehua~1 HUANG Yongping~2(~~1Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China ; ~2 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200032,China;~3 Graduate School of the Chinese Academy of Sciences,Beijing 100039, China)  
Inter-simple sequence repeat (ISSR) amplification was used to analyze genetic relationship among eri-silkworm (Philosamia cynthia ricini) germplasm. A total of 19 primers,containing simple sequence repeat(SSR) motifs, were tested for amplification on a panel of 8 varieties, and 9 of them could amplify distinct, reproducible bands. They amplified a total of 76 bands out of which 29 (37.66%) were polymorphic. The ISSR results suggested that dinucleotide motifs were more common in eri-silkworm than trinucleotide motifs and tetranucleotide motifs, and the poly(CA) motif was more abundant than the poly(CT) within the dinucleotide class. The genetic distances among eri-silkworm strains ranged 0.068 1~0.270 3, and the dendrogram was constructed based on their genetic distances using UPGMA method. Therefore, ISSR amplification proved to be a valuable method for determining genetic variability among eri-silkworm varieties. This efficient genetic fingerprinting technique would be useful for characterizing the large numbers of eri-silkworm strains held in national and international germplasm centers.
【Fund】: 国家科技基础平台子项目(编号2003DEA3N02903)。
【CateGory Index】: S885.2
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Chinese Journal Full-text Database 3 Hits
1 LIU Yan, MENG Zhi-qi, HE Li-hua, SHEN Wei-feng, NIU Bao-long, WENG Hon-gbiao (Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China);Compare of Two Methods for Extraction of Genomic DNA from Bombyx mori[J];Bulletin of Sericulture;2008-04
2 CAO Lan-Juan1 YANG Bao-Shan2 LI Jun1 LI Min1 WANG Zhuo1YANG Rui-Sheng1 QIN Li1(1 College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China;2Jinan University,Jinan 250022,China);Analysis of the Dictyoploca japonica With RAPD Technique[J];Science of Sericulture;2007-02
3 Weng Hongbiao1 Niu Baolong1 He Lihua1 Jiang Ping2 Meng Zhiqi1(1.Laboratory of Entomo-Molecular Biology,Zhejiang Academy of Agricultural Sciences Hangzhou 310021;2.Forest Pest Control and Quarantine Bureau of Zhejiang Province Hangzhou 310020);Genetic Diversity among the Dendrolimus punctatus Populations Sampled From Different Region of Zhejiang Province[J];Scientia Silvae Sinicae;2008-01
Chinese Journal Full-text Database 6 Hits
1 SHEN Li HUANG Yongping(Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032);Microsatellite DNA Markers and its Application Prospects in Sericulture Research[J];Acta Sericologica Sinica;2004-04
2 ZHONG Jin cheng, CHEN Zhi hua (Southwest Institute for Nationalities);Studies on DNA Molecular Markers and Animal Heterosis[J];JOURNAL OF SOUTHWEST NATIONALITIES COLLEGE(NATURAL SCIENCE EDITION);1999-01
3 ZUO Zheng-hong1,GUI Mu-yan2,WANG Xue-min2,CHEN Yuan-lin2 (1 Yueyang Heath School of Hunan,Yueyang 414000;2 Dept.of Bio.Xiamen University,Xiamen 361005,China);Application of RAPD Technique in Genetic Relationship Study of Silk Insect Ⅰ. Genetic Variance in Eri Silkworm[J];Hereditas(Beijing);2001-02
4 WANG Jian-bo(Key Laboratory of MOE for Plant Developmental Biology , Wuhan University ,Wuhan 430072,China);ISSR Markers and Their Applications in Plant Genetics[J];Hereditas(Beijing);2002-05
5 Jia Jizeng[WT〗 (Institute of Crrop Germplasm Resources, CAAS, Beijing 100081);Molecular Germplasm Diagnostics and Molecular Marker assisted Breeding[J];SCIENTIA AGRICUTURA SINICA;1996-04
6 LIANG Ming_Shan ZENG Yu ZHOU Xiang (Life Sciences College, Sichuan University,Chengdu 610064) HOU Liu_Ji LI Xia (Sichuan Tobacco Production and Trading Corporation,610000);Genetic Markers and Their Applications in Identifying Crop Cultivars[J];Chinese Bulletin of Botany;2001-03
Chinese Journal Full-text Database 10 Hits
1 SUN Jun-li et al (Northwest S&T University of Agriculture and Forestry, Yangling, Shanxi 712100);Characterization of SLA-DRA DRB Gene in Inbreed Line of WZSP Group by SSCP[J];Journal of Anhui Agricultural Sciences;2005-08
2 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance for Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-24
3 CHAI Li-juan~1,CHU Ming-xing~2,WEN Jie~2,CHEN Ji-lan~2,ZHAO Gui-ping~2,ZHENG Mai-qing~2,LIU Wen-zhong~1,ZHOU Zhong-xiao~1(1.College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801;2.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094);Analysis of AMPD1 Gene in Six Chicken Breeds by PCR-RF-SSCP[J];Journal of Anhui Agricultural University;2005-04
4 LI Rong-yu~(1,2),LIN Yi~1,CAI Yong-ping~1,ZHANG Jing~1,WEI Fengjuan~1(1.School of Life Science,Anhui Agricultural University,Hefei 230036; 2.Department of Microbiology and Immunology,Wannan Medical College,WuHu 241001);gDNA extraction and exploration of reaction system of RAPD analysis in wheat[J];Journal of Anhui Agricultural University;2006-01
6 WANG Yong\|Qing\ XU Lai\|Xiang\ ZHANG Zhi\|Bin (National Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, The Chinese Academy of Sciences Beijing\ 100080,China);The Standardization of RAPD[J];CHINESE JOURNAL OF ZOOLOGY;2000-04
7 CHEN Dong-Mei LIN Ying WANG Yan-Xia YANG Yu DAI Fang-Yin XIA Qing-You(College of Biotechnology,Institute of sericulture and system biology,Southwest University,Chongqing 400716,China);A simple method of isolating high quality Genomic dna from the silkworm[J];Newsletter of Sericultural Science;2007-02
8 Lu Cheng Zhao Aichun Zhou Zeyang Xiang Zhonghuai(The Key Sericultural Laboratory of Agricultural Ministry; College of Sericulture and Silk,Southwest Agricultural University,Chongqing 400716)Wan Chunling(Institute of Genetics,Chinese Academy of Science;AFLP Analysis of Chinese Mulberry Wild Silkworm ( Bombyx mandarina )and Domestic Silkworm( Bombyx mori )[J];Acta Sericologica Sinica;2001-04
9 Lu Cheng Chen Daojun Xiang Zhonghuai(The Key Laboratory of Sericulture of the Ministry of Agriculture;College of Sericulture and Silk,Southwest Agricultural University,Chongqing 400716)Banno Yutaka Fujii Hiroshi(Silkworm Genetic Division,Institute of;RAPD Analysis of Bombyx mandarina M.and Japanese (Fukuoka) Bombyx mandarina M.[J];Acta Sericologica Sinica;2002-01
10 Liu Yanqun 1,2 Lu Cheng 1 Xiang Zhonghuai 1( 1The Key Sericultural Laboratory of Agricultural Ministry,Southwest Agricultural University, Chongqing 400716; 2College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110161);RAPD Analysis of DNA Polymorphism of Antheraea pernyi[J];Acta Sericologica Sinica;2002-04
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 XIAO Jin-xue et al (College of Life Sciences,Yanan University,Yanan,Shaanxi 716000);Analysis on Biodiversity Research Status in China[J];Journal of Anhui Agricultural Sciences;2007-34
2 HE Gen-lin et al(The Key Lab of Animal Biology of Chongqing,Chongqing Normal University,Chongqing 400047);Comparative Research of Two Methods on DNA Extraction of Trichodinids subtilisa Lom.[J];Journal of Anhui Agricultural Sciences;2009-27
3 YANG Bao-Shan1,3,HOU Qing-Jun2,WANG Huan2,LI Xi-Sheng4,JIANG De-Fu4,LIU Yan-Qun2,,QIN Li2,(1.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China;2.College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110161,China;3.School of City Development,University of Jinan,Jinan 250002,China;4.Liaoning Sericultural Institute,Fengcheng,Liaoning 118100,China);Sequence variability of COI gene and genetic differentiation among the geographic populations of Caligula japonica(Lepidoptera:Saturniidae)in China[J];Acta Entomologica Sinica;2009-04
4 YANG Bao-Shan.1,2**,CAO Lan-Juan3,LI Jun3,LI Yi-Ren3,WANG Zhuo3,QIN Li.3***(1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China; 2. School of City Development University of Jinan, Jinan 250002, China; 3. College of Bioscience and Technology, Shenyang Agricultural University, Shenyang 110161, China);Genetic diversity assessment of Dictyoploca japonica from different areas[J];Chinese Bulletin of Entomology;2008-03
5 CAO Tian-Wen1,,ZHANG Min2,WANG Rui1,XUAN Shan-Bin1,WANG Ju-Ping1,GUO Ya-Ping2,MA En-Bo21 Institute of Plant Protection,Shanxi Academy of Agriculture Science,Taiyuan 030031,China2 College of Life Science and Technology,Shanxi University,Taiyuan 030006,China;RAPD analysis of genetic diversity of five populations of Pieris rapae(L.) in Sanxi Province,China[J];Acta Ecologica Sinica;2009-07
China Proceedings of conference Full-text Database 1 Hits
1 Li Guohong, Wang Wei, Zhao Tonghai (Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China);RAPD analysis on genetic diversity of seven populations of Hylobitelus xiaoi Zhang[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhong Jincheng Chen Zhihua(Dept.of animal husbandry and veterinary, Southwestern National College, Chengdu 610041, Sichuan);Review and prospects of studies on heterosis theory[J];JOURNAL OF YELLOW CATTLE SCIENCE;1996-04
2 Xia Qingyou;Zhou Zeyang;Lu Cheng; Feng Llchun; Xiang Zhongh ai(The SericuItural Key Laboratory of Ministry of of griculture,College of Sericulture & Silk,Southwest Agricultural University,Chongqing 6307l6,China);A STUDY ON CONDITIONS,AND REPEATABILITY AND INHERITANCE PATTERN OF RANDOM AMPLIFICATION POLYMORPHIC DNA(RAPD)IN F1 OF SILKWORM(BOMBYX MORI.)[J];ACTA SERICOLOGICA SINICA;1996-01
3 Zhu Cheng (Zhejiang Unirersity, Hangzhou 310029) Liu Feiyan (Hangzhou Botanical Garden, Hangzhou 310013);To apply RAPD in classification and differentiation of cultivar of Osmanthus fragrans Lour.[J];GUIHAIA;1999-02
4 Xia Qingyou\ Zhou Zeyang\ Lu Cheng\ Xiang Zhonghuai(The Key Sericultural Laboratory of Agricultural Ministry, College of Sericulture & Silk, Southwest Agricultural University\ Chongqing\ 400716);MOLECULAR PHYLOGENETIC STUDY ON THE RACIAL DIFFERENTIATION OF BOMBYX MORI BY RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD) MARKERS[J];ACTA ENTOMOLOGICA SINICA;1998-01
5 Zhang Heng-qing 1 An Li-jia 2 Zu Yuan-gang 1(1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry Univ., Harbin 150040) (2. Chemical Institute, Dalian Science and Technology Univ., Dalian 116001);EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS[J];;1999-02
6 Xia Ming Luan Fei-shi Li Jing-peng(Northeast Agricultural University, Harbin 150030);STUDY ON INFLUENCING FACTORS OF RAPD AND OPTIMIZATION OF RAPD EXPERIMENT CONDITIONS[J];;1999-02
7 Hu Guoyuan The institute of applied microbiology Hubei institute for nationality,Enshi Hubei 445000; Zhu Lanbao Zhou YuLin The institute of applied mycology Huazhong agriculutural Univetsity,Wuhan Hubei 430070;RESEARCH ON SCREENING OF ESTERASE ISOZYME MARKER AMONG STRAINS IN FLAMMULINA VELUTIPES[J];JOURAL OF BIOLOGY;1999-01
8 ZHANG Feng SONG Wen Qin CHEN Rui Yang (Biology Department,Nan Kai University,Tianjin, 300071);AFLP SILVER STAINING METHOD DETECTING THE POLYMORPHISM IN PLANT GENOME[J];Chinese Journal of Coll Biology;1999-02
9 Xia Qingyou;Zhou Zeyang;Lu Chen;Xiang Zhonghuai(The Key Sericultural Laboratory of Agricultural Ministry,College of Sericuhure,Southwest Agricultural University,Chongqing 630716);ISOLATION OF RAPD MARKERS FOR Y,NL ̄1 AND Z CHROMOSOME IN BOMBYX MORI[J];JOURNAL OF SOUTHWEST AGRICULTURAL UNIVERSITY;1996-02
10 CAI Cong-Li, WANG Jian-Bo, JING Run-Chun, ZHU Ying-Guo (College of Life Sciences, Wuhan University, Wuhan 430072, China);RAPD Analysis of the Genome Evolution in Allopolyploid Species in Aegilops[J];Acta Genetica Sinica;2001-02
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