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《Acta Sericologica Sinica》 2005-03
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Genetic Diversity Among Philosamia cynthia ricini Strains Revealed by ISSRs

HOU Chengxiang~1 LI Muwang~1,2,3 MIAO Xuexia~2 XU Anying~1SUN Pingjiang~1 ZHANG Yuehua~1 HUANG Yongping~2(~~1Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China ; ~2 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200032,China;~3 Graduate School of the Chinese Academy of Sciences,Beijing 100039, China)  
Inter-simple sequence repeat (ISSR) amplification was used to analyze genetic relationship among eri-silkworm (Philosamia cynthia ricini) germplasm. A total of 19 primers,containing simple sequence repeat(SSR) motifs, were tested for amplification on a panel of 8 varieties, and 9 of them could amplify distinct, reproducible bands. They amplified a total of 76 bands out of which 29 (37.66%) were polymorphic. The ISSR results suggested that dinucleotide motifs were more common in eri-silkworm than trinucleotide motifs and tetranucleotide motifs, and the poly(CA) motif was more abundant than the poly(CT) within the dinucleotide class. The genetic distances among eri-silkworm strains ranged 0.068 1~0.270 3, and the dendrogram was constructed based on their genetic distances using UPGMA method. Therefore, ISSR amplification proved to be a valuable method for determining genetic variability among eri-silkworm varieties. This efficient genetic fingerprinting technique would be useful for characterizing the large numbers of eri-silkworm strains held in national and international germplasm centers.
【Fund】: 国家科技基础平台子项目(编号2003DEA3N02903)。
【CateGory Index】: S885.2
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