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《Science of Sericulture》 2006-01
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Analysis of 2D-PAGE Patterns of Proteins in Posterior Silkgland From Different Silkworm Varieties on 4~(th) Day in 5~(th) Instar

LU Hua-Yun~(2) YE Jian~(1) LI Jian-Ying~(1) ZHONG Bo-Xiong~(1*) (~(1) Laboratory of Molecular Biology of Silkworm and Honeybee,College of Animal Science,Zhejiang University,Hangzhou 310029,China;~(2) Hangzhou Popularization Station for Fine Breed of Special Local Products,Hangzhou 310002,China)  
Using two-dimensional electrophoresis of protein and image analysis system,we discovered that protein constituents in the posterior silkgland on 4~(th) day in 5~(th) instar are apparently different for different silkworm varieties.Comparing with those in silkworm variety with the middle,low yield cocoon,we found that there existed many different protein spots in silkworm variety with the high yeild cocoon.This suggested that the transcription and translation of fibroin gene might be involved in a complex regulation system with a great deal of regulation points,and the yeild cocoon in different varieties may be related with different regulation points or factors.
【Fund】: 国家重点基础研究发展计划973项目(编号2005CB121003);; 浙江省科技厅项目(编号2004C32048)
【CateGory Index】: S881
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