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《Science of Sericulture》 2006-02
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Studies on Factors Affecting Callus Induction and Adventitious Buds Differentiation From Leaf Culture in Mulberry

WANG Yan-Wen~(1,2) HUANG Yan-Hong~(1) LU Guo-Bing~(2) WANG Jun-Ni~(2) MU Zhi-Mei~(1,2*)(~(1)College of Animal Science & Technology,Sichuan Agricultural University,Yaan Sichuan 625014,China;~(2)College of Forestry,Shandong Agricultural University,Taian Shangdong 271018,China)  
Using orthogonal experiment design,the effect of Thidiazuron(TDZ) and α-Naphthalencacetic acid(NAA) content in the medium, effect of the order of leaf,the age of leaf and the varieties of mulberry on the leaf callus induction was investigated.Meanwhile,the effect of 6-benzylaminopurine(BA),indole-3-acetic acid(IAA) and sugar on callus differentiation was also studied.The results showed that the best condition for callus induction is: age of the mulberry cultured in vitro,21 d;medium,MS(Murashige Skoog medium)+1.0 mg/L TDZ+0.2 mg/L NAA;and that the medium for adventitious bud differentiation of the callus is: MS+3.0 mg/L 6-BA+0.1 mg/L IAA,fructose,2%.Under these conditions,the leaf callus induction rate and adventitious bud differentiation rate for Shan 305 reached 93.9% and 100% respectively.
【Fund】: 山东省农业良种产业化工程项目(编号2002228)
【CateGory Index】: S888.32
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