Study on the Esterase Isozyme of Tussah Silkworm,Antheraea pernyi
TONG Zhen-Xiang WANG Feng-Cheng JI Wan-Jie PIAO Mei-Lan(The Sericultural Research Institute of Liaoning Province, Fengcheng Liaoning 118100, China)
Esterase isozyme of Tussah silkworm was investigated using PAGE method concerning different developing stages, organs and varieties. Activity and the kinds of esterase in the midgut varied among different developing stages. The activity and numbers of esterase loci were both higher in massive ingestion stage than that in other stages, suggested that the expression of esterase isozyme related to the development of Tussah silkworm. The kinds and activity of esterase varied greatly among different organs of Tussah silkworm larva. Esterase in fat body and midgut had higher activity and more kinds than that in other organs, indicated that the activity of esterase isozyme was closely related to the function of specific organ. The esterase isozymes were expressed differently among different varieties, but all the varieties shared a common esterase loci at Rf 0.65. The result of clustering analysis with UPGMA method showed that 33 Tussah varieties were classified into 4 groups, most varieties had closer relationship with their female parents, indicated that female parents play an important role in line separation breeding. The result suggested that more attention should be paid on the selection of female parents during the process of hybrid breeding. For some of the varieties bred by system separation method, part of their characters that come from their parent line may tapered gradually during long term intensive selection while some specific characters strengthened, resulted in the far blood relationship with their parents.
【CateGory Index】： S885.1