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《Science of Sericulture》 2008-02
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RAPD Analysis of Blepharipa tibialis From Different Areas and Seasons

WANG Zhuo1 CAO Lan-Juan1 LIU Zhen-Lin2 JIANG Yi-Ren1YANG Rui-Sheng1 ZHANG Tao1 QIN Li1(1Biological Science & Technology College, Shenyang Agricultural University, Shenyang 110161, China;2 Mudanjiang Sericulture Station of Heilongjiang Province, Mudanjiang Heilongjiang 157000, China)  
The genetic diversity of Blepharipa tibialis from different areas and seasons was analyzed by RAPD method in this paper. Eleven random primers were selected to amplify the genomic DNA of 14 samples. A total of 140 bands were amplified, among which 128 bands (91.43%) were polymorphic. The results showed polymorphism of Blepharipa tibialis was high. The genetic distance among 14 samples ranged from 0.021 4 to 0.514 3. According to UPGMA cluster analysis, a DNA molecular dendrogram was developed from 14 samples, which were divided into 2 groups. One of which was parasitized in Dictyoploca japonica (unnamed) and the other was parasitized in Antheraea pernyi. Blepharipa tibialis from Henan province was clustered into one group, and Blepharipa tibialis in Northeast was clustered into another group.
【Fund】: 辽宁省科技攻关项目(编号2001209001)
【CateGory Index】: S885.1
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