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《Science of Sericulture》 2008-04
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Construction of the Core Collection of Morus multicaulis Perr.Germplasm Resources From Shandong and Hebei Based on ISSR Molecular Markers

CHEN Jun-Bai1 HUANG Yong1 ZHANG Lin1,2,3* ZHAO Wei-Guo1,2 PAN Yi-Le1,2(1School of Biotechnology and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China;3College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)  
In order to conserve and utilize mulberry germplasm resources more efficiently,we developed the core collection of 46 Morus multicaulis Perr.germplasm resources from Shandong and Hebei in this paper.By using 15 selected ISSR primers,98 DNA bands were amplified from them.The result of 98 ISSR markers and the dendrogram of clustering,stepwise clustering and random methods were used to screen the core collection.Based on the parameters of 4 sample groups,including the number of polymorphic loci,percentage of polymorphic loci,and the number of alleles observed,effective number of alleles,Nei's gene diversity and Shannon's information index,11 samples were chosen as core collection.The parameters between initial collection and core collection were T-tested by using SPSS software.The results showed that the number of core collection samples is only 23.91% of initial collection,but the parameters above-mentioned of the core collection reached 89.02%,89.03%,95%,102.24%,103.99%,and 101.26% of initial collection,respectively.Those results indicated that the core collection could represent initial collection remarkably.
【Fund】: 国家科技基础条件平台工作子项目(编号2005DKA21002-09)
【CateGory Index】: S888
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