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《Science of Sericulture》 2008-04
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Cloning of the CYP 9A22 of Cytochrome P450 Genes From the Silkworm,Bombyx mori and Induced Transcription of CYP 9A22 in Fat Body and Midgut

ZHAO Hua-Qiang1 WANG Dong1 LI Bing1,2 XU Ya-Xiang1,2 SHEN Wei-De1,2*(1School of Basic Medicine and Biological Sciences,Soochow University,Suzhou Jiangsu 215123,China;2Institute of Sericulture,Soochow University,Suzhou Jiangsu 215123,China)  
Cytochrome P450 has been proved to be involved in growth and development,environment adaptation and insecticide resistance in insect.In order to illustrate the inducement effect of cypermethrin treatment on the transcription of cytochrome P450 genes in Bombyx mori,CYP9A22(GenBank accession number: EF535804) from 5th instar larvae was cloned by methods of RT-PCR and RACE.The coding region of CYP9A22 cDNA is 1 596 bp,which encodes 531 amino acid residues with the predicted molecular weight of 61 kD and isoelectric point of 7.71.Alignment result of the cDNA with the silkworm genomic sequences revealed that there are 9 introns in this gene.The deduced amino acid sequence showed 62.3%identity to CYP9A14 from Helicoverpa armigera.The inducement effect of cypermethrin treatment on the transcription of CYP9A22 were determined by semi-quantitative RT-PCR method.The results showed that the transcription level was higher in fat body than in midgut of the 5th instar larvae.The induced transcription level in fat body by the cypermethrin treatment for 24 h was 3.1 times of the control,suggested that over-transcription level of CYP9A22 maybe involved in pyrethroid resistance of Bombyx mori.
【Fund】: 国家重点基础研究发展计划“973”项目(编号2005CB12-1005);; 国家高技术研究发展计划“863”项目(编号2006-AA10A118)
【CateGory Index】: S881.2
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