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《Science of Sericulture》 2009-01
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Molecular Cloning of Glutathione S-transferase Theta Gene From Antheraea pernyi and Expression Analysis in Silk Glands of the Fifth Instar Larvae

XU Shu-Rong1 LIU Dan-Mei1,2 LI Wen-Li1(1School of Environmental & Biological Science & Technology,Dalian University of Technology,Dalian Liaoning 116023,China;2School of Agriculture,Eastern Liaoning University,Dandong Liaoning 118001,China)  
Glutathione S-transferase(GST) is an enzyme with detoxification function.In order to study functions of glutathione S-transferase theta(GSTT) during preparation and synthesis of fibroin in Antheraea pernyi,expression of GSTT in silk glands of the fifth instar larvae was analyzed.Based on the nucleotide sequence of GSTT gene in Bombyx mori,the cDNA sequence of GSTT from A.pernyi was amplified.The open reading frame(ORF) of the GSTT cDNA from A.pernyi is 651bp long and encodes 216 amino acid residues(GenBank accession No.EU541490).The deduced amino acid sequence of GSTT shared 89% identity with B.mori GSTT.Quantitative real-time PCR revealed that expression of GSTT reached the highest level at the 4th day and then declined.This increased expression during the synthesis of fibroin suggests that GSTT is needed to remove excessive amino acids during fibroin synthesis so as to eliminate the toxic effect of excessive amino acids.
【Fund】: 辽宁省自然科学基金项目(编号20062182)
【CateGory Index】: S885.1
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