Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Science of Sericulture》 2009-04
Add to Favorite Get Latest Update

Studies on Pretreatment of Eggs From Bivoltine Silkworm Varieties(Strains) for Micro-injection Transgene

TANG Shun-Ming1,2 SHEN Xing-Jia1,2 ZHAO Qiao-Ling1,2 ZHANG Yang2 ZHANG Guo-Zheng1,2 GUO Xi-Jie1,2(1The Key Laboratory of Genetic Improvement of Silkworm and Mulberry,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China;2Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China)  
The silkworm,Bombyx mori,is a species of insect with diapause during egg stage.The voltinism of bivoltine silkworm variety(strain) is regulated by temperature and illumination in its parental egg stage.From a number of bivoltine varieties(strains) of which eggs were induced to become non-diapause by control of temperature at 17~18 ℃ during egg incubation,one practical silkworm variety Qiufeng with excellent economic characters and one mutant strain P33 were selected.Their non-diapause egg batch percentages were 96.4% and 89.6%,and practical hatching abilities of good eggs under normal incubation condition were 96.11% and 98.58%,respectively.These results have resolved the difficulty that diapause eggs can not hatch directly after micro-injection in transgenic studies.In addition,the efficiency of dipping eggs in mixture of 20% hydrochloric acid and 2% formaldehyde solutions for activating diapause eggs and the effect of surface disinfection with 70% ethanol for non-diapause eggs before micro-injection were investigated.The results showed that the former could provide a hatching ability higher than 96%,and the latter could shorten the egg pretreatment duration significantly.
【Fund】: 国家重点基础研究发展计划“973”项目(编号2005CB12-1004);; 国家高技术研究与发展计划“863”项目(编号2007AA100504);; 江苏省高校自然科学基金项目(编号07KJD230052)
【CateGory Index】: S882
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Yongqiang Xu Mengkui(Department of Sericultural and Apiculture,College of Animal Sciences,Zhejiang University,Hangzhou 310029)He Xiuling Xia Jianguo(Sericultural Research Institute,Zhejiang Academy of Agricultural Sciences,Ha;Studies on Parthenogenesis of Commercial Silkworm Races[J];Acta Sericologica Sinica;2001-01
2 HUANG Junting(The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018);Studies on the Molecular Mechanism of Diapause in the Silkworm,Bombyx mori[J];Acta Sericologica Sinica;2003-01
3 ZHANG Ye-Shun1,2 XIA Ding-Guo2 ZHANG Guo-Zheng1,2 Tang Shun-Ming2SHEN Xing-Jia2 ZHAO Hao-Qin2 GUO Xi-Jie1,2(1Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China;2Key Laboratory of Silkworm Biotechnology,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China);The Improvement on the Sperm Mediated Gene Transfer System for the Silkworm,Bombyx mori[J];Science of Sericulture;2007-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Deng yongjin et al(Sericulture Research Institute,Anhui Academy of Agricuitural Sciences,Hefei 230061);Application of Transgenic Techniques to Breeding of New Variety of Bombyx Mori[J];Anhui Agricultural Science Bulletin;2007-23
2 ZHANG Lei et al (Institute of Sericulture, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230061);Study on Control Efficacy of Fangjiangling Ⅱ on Silkworm Mycosis[J];Journal of Anhui Agricultural Sciences;2007-26
3 QIU Zhi-yong et al(Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu 212018);Factor Analysis of Stress-resistance and Some Variety Character of Silkworm[J];Journal of Anhui Agricultural Sciences;2008-20
4 DENG Yong-jin et al (Sericulture Research Institute,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031);Discussion on Transgenic Technology and the Breeding of New Silkworm Variety[J];Journal of Anhui Agricultural Sciences;2008-25
5 WANG Ping,LI Yi-ren,LI Gui-fang(Testing Center of Quality of Sericultral Products,Ministry of Agriculture,the Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018);Comparison of the rearing effects of different artificial diets for young silkworm during different instars[J];Journal of Anhui Agricultural University;2007-01
6 Ren Yinghong Shen Xiaofang (Xichang Academy of Agriculture,Xichang Sichuan,615013);Botanic Comparison Experiments for Appraising the Quality of Fine Varieties of Mulberry Leaves in Panxi Area[J];JOURNAL OF CHONGQING TEACHERS COLLEGE(NATURAL SCIENCE EDITION);1999-02
7 YU Rong-feng, WANG Heng-sheng, HU Fu-bo, FANG Zheng (Zhejiang Chunan Cocoon and silk Company, Chun'an 311700, China);Preliminary Report on Effect of Cocooning Conditions on Cocoon Quality[J];Bulletin of Sericulture;2004-04
8 Zhang Xue-ming(The Parent Egg Production Station of Zhejiang Province,Hangzhou 310023,China);The Research Advancement of Silkworm Artificial Diet Rearing[J];Bulletin of Sericulture;2006-03
9 FU Shu-qing1,LI Jun2,SHI Zhou-ming3,CHEN Yu-yin2(1.Shaoxing Extension Station of Special Economic Products,Shaoxing 312000,China; 2.College of Animal Sciences,Zhejiang University,Hangzhou 310029,China;3.Dingzhai Town Government,Shangyu 312300,China);A Preliminary Discussion about Biological Significance of Sex-Balance in Larval Stage of Silkworm Bombyx mori[J];Bulletin of Sericulture;2006-04
10 LIU Yan, WENG Hong-biao, HE Li-hua, NIU Bao-long, SHEN Wei-feng, QI Xiao-peng, MENG Zhi-qi (Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China);In Silico Cloning of the BmEDF Gene in Silkworm, Bombyx mori[J];Bulletin of Sericulture;2007-03
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Huang Jianhui;He Kerong;Xia Jianguo;Progresses of Research on Parthenogenesis of the Silkworm (Bombyx mori)[J];BULLETIN OF SERICULTURE;1997-01
2 Wang Yongqiang Xia Jianguo Yao Lusong (Institute of Sericulture, Zhejiang Academy Of Agriculture Sciences) Xu Mengkwei (Department of Sericultural Science, Zhejiang University);Study on the Optimum Conditions of Silkworm Parthenogenesis Induced by Hot Water Treatment[J];Bulletin of Sericulture;1998-04
3 Fang Ai Xu Anying Chen Suqian Huang Junting (The Sericultural Research Institute, Chinese Academy of Agricultural Sciences);STUDIES ON THE CHROMSOME ENGINEERING AND ITS APPLICATION IN BOMBYX MORI IV. Study on the Parthenogenesis in the Silkworm, Bombyx Mori L.[J];Acta Sericologica Sinica;1989-04
4 CAO Guang-Li XUE Ren-Yu HE Ze ZHENG Xiao-Jian SHEN Wei-De GONG Cheng-Liang~*(College of Life Sciences,Soochow University,Suzhou Jiangsu 215123,China);Research on hGM-CSF Transgentic Silkworm With piggyBac Transposon[J];Science of Sericulture;2006-03
5 Chen Xiu, Zhang Feng *, Zhao Yun *, Qi Guorong *, Lu Changde *, Huang Junting (Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018) ( *Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 2000;Application of Luciferase Report Gene in Transgenic Silkworm[J];HIGH TECHNOLOGY LETTERS;2000-01
6 JIN Ying (Institute of Laser Life Science, South China Normal University, Guangzhou, 510631);Progress on Green Fluorescent Protein Research[J];ACTA LASER BIOLOGY SINICA;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved