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《Science of Sericulture》 2009-04
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Studies on Pretreatment of Eggs From Bivoltine Silkworm Varieties(Strains) for Micro-injection Transgene

TANG Shun-Ming1,2 SHEN Xing-Jia1,2 ZHAO Qiao-Ling1,2 ZHANG Yang2 ZHANG Guo-Zheng1,2 GUO Xi-Jie1,2(1The Key Laboratory of Genetic Improvement of Silkworm and Mulberry,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China;2Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China)  
The silkworm,Bombyx mori,is a species of insect with diapause during egg stage.The voltinism of bivoltine silkworm variety(strain) is regulated by temperature and illumination in its parental egg stage.From a number of bivoltine varieties(strains) of which eggs were induced to become non-diapause by control of temperature at 17~18 ℃ during egg incubation,one practical silkworm variety Qiufeng with excellent economic characters and one mutant strain P33 were selected.Their non-diapause egg batch percentages were 96.4% and 89.6%,and practical hatching abilities of good eggs under normal incubation condition were 96.11% and 98.58%,respectively.These results have resolved the difficulty that diapause eggs can not hatch directly after micro-injection in transgenic studies.In addition,the efficiency of dipping eggs in mixture of 20% hydrochloric acid and 2% formaldehyde solutions for activating diapause eggs and the effect of surface disinfection with 70% ethanol for non-diapause eggs before micro-injection were investigated.The results showed that the former could provide a hatching ability higher than 96%,and the latter could shorten the egg pretreatment duration significantly.
【Fund】: 国家重点基础研究发展计划“973”项目(编号2005CB12-1004);; 国家高技术研究与发展计划“863”项目(编号2007AA100504);; 江苏省高校自然科学基金项目(编号07KJD230052)
【CateGory Index】: S882
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