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《Science of Sericulture》 2010-02
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Isolation and Identification of Mulberry Rhizospheric Silicate Bacteria and Determination of Their Potassium-releasing Activities

WU Fan1,2 LIU Xun-Li1 ZHANG Nan1 ZHANG Sha-Sha1 GUO Hui1 ZHANG Ben-Feng1 QIU Nian-Quan1(1College of Forestry,Shandong Agricultural University,Taian Shandong 271018,China;2Industrial Crops Institute of Hubei Academy of Agricultural Sciences,Wuhan 430064,China)  
29 bacterial isolates that had potassium-releasing activities were obtained from mulberry rhizospheric soil through cultivation with selective media.Analysis of repetitive-element PCR(rep-PCR)genomic DNA fingerprinting identified 24 silicate bacteria from them.Determination of their potassium-releasing activities indicated that 7 isolates,i.e.FK2,FK3,FK8,FK11,FK4',FK23 and PK18,had high potassium-releasing activities,among which FK2 had the highest potassium-releasing activity.Its available potassium increment reached 41.79%.Observations on morphological features of bacterial colonies and phylogenetic analysis of 16S rDNA sequences to the 7 isolates with high potassium-releasing activity revealed that FK2 belonged to Pseudomonas sp.,FK3 and FK23 belonged to Sinorhizobium sp.,FK11 and FK8 belonged to Rhizobium sp.,FK4' belonged to Mesorhizobium sp.,and PK18 belonged to Flexibacter sp.
【Fund】: 山东省科技攻关计划项目(No.02201010102)
【CateGory Index】: S888.2
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