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《Science of Sericulture》 2016-06
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Variations in Germination Rate and Biochemical Property of Mulberry Seeds from Three Different Geological Regions Under Salt Stress

Ban Yueyuan;Fang Rongjun;Du Wei;Yin Chaorui;Cao Xu;Liu Li;Cheng Jialing;The Sericulture Research Institute,Jiangsu University of Science and Technology;Research Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences;  
To enrich information of mulberry salt resistance mechanism,and to select mulberry germplasm resources with strong salt tolerance as breeding material or for direct application in saline land treatment,mature seeds of mulberry( Morus L.) were collected from Hetian District of Xinjiang Uygur Autonomous Region,Harbin of Heilongjiang Province and Haian County of Jiangsu Province respectively and used as experimental materials to study the effects of 1- 9 g / L Na Cl solution on relative germination rate,content of osmotic regulative substances and antioxidant enzyme activity of germinated seedlings. Real-time fluorescent quantitative PCR was performed to analyze the expression patterns of peroxidase POD1 and superoxide dismutase sod C genes in seeds collected from Harbin. The results showed that the relative germination rates of seeds from three different regions decreased with the increase of Na Cl concentration. Under the same Na Cl stress condition,mulberry seeds from Hetian had the highest relative germination rate. Proline content and soluble protein content in germinated seed-lings increased with the increase of Na Cl concentration. Soluble sugar content and POD activity in the seedlings increased initially and then declined with the increase of Na Cl concentration. SOD activity in the seedlings decreased continuously with the increase of Na Cl concentration. Transcriptional levels of POD1 and sod C genes were not completely consistent with their enzyme activity. These results confirm that mulberry trees have the potential for further development and utilization in saline land treatment,and salt tolerance of mulberry seeds from Hetian District is the best. The obove results also show that the transcription levels of POD1 and sod C genes should not be used as the only index to judge salt tolerance of mulberry.
【Fund】: 现代农业产业技术体系建设专项(No.CARS-22)
【CateGory Index】: S888.2
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