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《ACTA SERICOLOGICA SINICA》 1994-03
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STUDIES ON MULBERRY PROTOPLAST ISOLATION TECHNIQUES

Chen Ai-yu Wang Yong Ni Guo-fu (Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,China )  
Mulberry cotyledons,young leaves,suspension cells and calli were used for proto-plast isolation studies. The results showed that :1) Under appropriate enzyme concentra-tion,the release speed and yield of mulberry protoplasts was in the order of cotyledonsyoung leaves suspension cellscalli;2) The optimum osmotic concentration forenzyme solution was 0.5~0.6M tnannitol;3) Pretreatment to young leaves increasedprotoplast yield by 12. 9 times.
【Fund】: 国家自然科学基金
【CateGory Index】: S888.31
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