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Zhu Xiangrui Bao Huijin(Department of Sericulture,Zhejiang Agricultural University, Hangzhou 310029,China)  
In this investigation,the SOD was extracted from terminal bud leaves,the 5thleaves,the 10th leaves and waste leaves in the litter of fifth instar silkworm of fourdifferent mulberry varieties,Tongxiangqing,Tantouheyebai,Jiantouheyebai andHusang 197 by two methods,namely,high speed and cold centrifugation, and normalspeed and room temperature centrifugation. SOD activity was determined by NBT lightreducing reaction,and the iosenzyme of the SOD was analyzed by polyacrylamideelectrophoresis.The experiment showed that activity of the SOD extracted with twodifferent methods has no significant difference,and that from the same leaf order ofdifferent varieties has no significant difference either,But that from the different leaforder of the same varieties has significant difference.And the SOD activity from thewaste mulberry was nearly equal to that of the 10th leaves.The experiment also showedthat the mulberry leaves have four kinds of iosenzyme of the SOD.
【CateGory Index】: S888.2
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