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《ACTA SERICOLOGICA SINICA》 1999-01
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Effects of Polypeptide Inhibitory Needle (PIN) on Activity of Esterase A4 in Vitro

Xu Shiqing Zheng Biping Chen Xilin Sima Yanghu Yu Xiaohua(Sericultural college,Suzhou University,Suzhou 215151,China)Kai Hidenori(Faculty of Agricultural,Tottri University,Koyama,Tottri,680 Japan)Xu Junliang(Department of Sericulture  
Esterase A4 (Ease A4) and its polypeptide inhibitory needle (PIN) were purified from diapause eggs of C108 Bombyx mori 2 days after oviposition.Effects of PIN on ATPase activities of Ease A4 was investigated in vitro .The Ease A4 was incubated with PIN for 2.5~13 days at 5℃ or 25℃.After removing of the PIN by centricon 10,the Ease A4 was chilled at 5℃.When the Ease A4 was incubated at 5℃,the ATPase active peaks appeared at 12~14 days after removing of the PIN.While at 25℃,the peaks appeared at 12~14 days plus the incubation duration.By using the method of matrix assisted laser desorption ionization mass spectrometry (MALDIMS),the complex of purified Ease A4 and synthesized PIN was found for the first time.
【CateGory Index】: S881.2
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