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《Journal of Tea Science》 1986-01
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Studies on the Physiology of Cold Resistance of Tea Plant——The Enzymes and Cell Membrane Permeability with Respect to the Cold Resistance of Tea Plant

Huang Huatao(Department of Agronomy,South China Agricultural University,Guangzhou)Liu Zusheng;Zhuang Wanfang(Department of Tea,Zhejiang Agricultural University,Hangzhou)  
Changes of the activities of catalase and peroxidase and the peroxidase isozy-mograms in the leaves of 10 varieties with different cold resistant ability were determinedin natural condition in Hangzhou.Changes of cell membrane permeability and theability of tea leaves to reduce TTC were also determined at low temperature throughartificial treatment.The main results of studies are as follow:Activities of catalase and peroxidase in the leaves of tea plants were stronger inWinter than in Autumn,and the band number of peroxidase isozymograms increasedfrom Autumn to Winter.Hardy varieties showed peaks of catalase activity earlier duringoverwintering and showed more vigrous activity of catalase and had more peroxidaseisozymes in the severe cold period.The degree of change of the cell membrane permeability varied with the temperatureand the time of treatment,stage of overwintering and varieties.It was showed that thechanges of cell membrane permeability of various varieties were obviously different atsome given temperatures.According to this fact,detached leaves are suggested to beexposed to the temperature -6℃ for 3—6 hours before using the methods of electricconductivity and K~+ exosmosis to detect the cold resistance of tea plant around novemberin Hangzhou.The ability of tea leaves to reduce TTC decreased as the temperature was loweredand showed a negative correlation with the cell membrane permeability.
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