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Comparative Studies on Isozymograms and Activities of Some Enzymes in Organs of Tea Shoot in Autumn Season

Huang Huihau;Chen Xinyan;Chen Guoben;Wang Jianguo(Tea Laboratory of Hunan Agricultural College,Changsha)  
Some studies on the isozymes of peroxidase,polyphenol oxidase,esterase and theactivities of peroxidase,polyphenol oxidase in six parts(including one bud and fiveleaves)of growing tea shoot in autumn season were conducted.The results are asfollows:15 bands of peroxidase,6 bands of polyphenol oxidase,and 9 bands of esterasewere detected.There were three kinds of peroxidase isozymograms,two kinds ofpolyphenol oxidase isozymograms and the esterase isozymogram showed no differenceamong the bud and leaves.The band PX_2 of peroxidase was found as the marker bandin the first leaf to the fourth leaf.The sum activity of peroxidase and it's band PX_(13)increased as the organ changedfrom bud to the fifth leaf and the activity of band PX_(15)showed two highest peaks inthe second leaf and the fifth leaf.The activities of polyphenol oxidase in bud and thethird leaf were lower than those in other leaves.The bands in the polyphenol oxidaseisozymogram showing difference in activity among the bud and leaves were PL_1,PL_3and PL_8.In the isozymogram of esterase the activities of bands ES_(13)ES_(14)significantlydecreased as the organsochanged from bud to the fifth leaf,showing opposite to thatof peroxidase.These results indicated that the peroxidase isozymograms,polyphenol oxidasewere correlative with the development of tea shoot.And some active bands such asPX_2,PX_(13),PX_(15),PL_1,PL_3,PL_8,ES_(13),ES_(14)of the three kinds of the enzymesindecated there were differences in organs in metabolize and ageing of the leavse of teashoot.
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