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《Journal of Tea Science》 1993-01
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Study on the Ichthyism Activity of Tea Saponin and Its Application Ⅴ.Haemolysis and Ichthyism Activity of Tea Saponin

Zhu Quanfen;Xia Chunhua;Fan Xingtu;Liu Rongxiang;Tian Jiehua;Wang Lu;Tang Liping(Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou310008)  
Results of microscopical observation on the bloods which were partedfrom the bodies of fish,prawn,chicken and human,and the bloods whichwere in the bodies of fish and prawn showed that tea saponin had haemolysisto red blood cell but hadn't haemolysis to white blood cell and prawn's blood.The ichthysis activity to red blood cell expressed that tea saponin destroyedthe membrane of the red blood cell then the cell isclusion was flowed out.The ichthyism activity of tea saponin expressed that tea saponin destroyedthe epithelioid cell of fish gill then went into the gill and the hart,at theend,led the red blood cell to haemolysis,but tea saponin did not act onprawn.After determined the relationship of the haemolytic ratio and thetime-dilution ratio curve with tea saponin concentration,the results showedthat the haemolytic ratio curve was‘S’type in shape.It meant that thehaemolytic rate was not uniform.It was necessary for 30 μg purity teasaponin to haemolysis 1 ml standard haemocyte suspension.The haemolyticindex of tea saponin was 100000.
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