Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Tea Science》 2001-01
Add to Favorite Get Latest Update

Study on the Method of HPLC Analysis of Catechins in Tea Polyphenols

YU Hai ning 1,SHEN Sheng rong 1,ZANG Rong chun 2,FENG Ping 1[WT6BX] (1.Department of Tea Science,Zhejiang University; 2.Center of Analysis and Test,Zhejiang University,Hangzhou 310029,China )[WT5”HZ]  
A kind of analytical method by high performance liquid chromatography(HPLC) for tea catechins with ingradient elution system was put forward In the meantime,the method was investigated in the methodological aspect through separating and determining the main four catechins (EC,ECG,EGC,EGCG) of tea polyphenols The optimum chromatographic conditions were as follows:ODS column (5 μm,4 6×250 mm),water/acetonitrile/ethylacetate (v/v/v,8 6∶12∶2) as mobile phase (add H 2SO 4 to adjust pH 3 0~4 0),UV detector (280 nm),flow rate:1 0 ml/min Moreover,the precision and stability of the method was discussed
【Fund】: 浙江省科委大型仪器分析测试基金资助项目! (编号 :9912 9)
【CateGory Index】: O657.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 LI Qiong1,2,RUAN Jian-yun1(1.Tea Research Institute of Chinese Academy of Agricultural Sciences,Hangzhou 310008,China;2.Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China);Effects of Fluoride on the Metabolism of Tea Quality Components[J];Journal of Tea Science;2009-03
2 Li Meng Wang Hua-yan Hu Wen-xiang (Department of Chemistry,Capital Normal University,Beijing 100037,China);HPLC Determination of Catechins and Caffeine in Tea Polyphenols[J];Chinese Journal of Medicinal Guide;2007-06
3 CHEN Hua-cai~(1,2),LU Jin~1,CHEN Xing-dan~2,LIU Hui-jun~1 (1.Chinese Jiliang University,Hangzhou 310018,China;2.State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China);Near infrared spectroscopic model for determinating total catechins in tea polyphenol powder based on radical basis function network[J];Optics and Precision Engineering;2006-01
4 LI Zi-yong,YU Zong-yuan,XU Xiao-jun,YOU Qing-liang,ZHANG Hong-min(Hubei Research Institute of Chemistry,Wuhan 430074,China);Study on the Relationship of Tea Polyphenol Content and Catechin Content[J];Chemistry & Bioengineering;2007-11
5 LI Jing,LI Jing-yin,GUO Yu-feng,DUAN Hui-min,WANG Wei-xiao(College of Sciences,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China);Electrocatalytic action of catechin at modified electrode of multi-walled carbon nanotube[J];Journal of Hebei University of Science and Technology;2006-04
6 LU Tao1,LAN Xianqiu1,ZHU Bin1,LUO Anlin2,SONG Hang1(1Department of Pharmaceutical and Biological Engineering,Sichuan University,Chengdu 610065,Sichuan,China;2Sichuan Hang Jia Biological and Pharmaceutical Technology Company,Limited,Chengdu 611430,Sichuan,China);Determination of catechins in different tea polyphenols and comparison of EGCG isolation by column chromatography[J];Chemical Industry and Engineering Progress;2008-05
7 LI Mingyu, ZENG Qingxuan, FENG Changgen, SUN Weina (State Key Laboratory for Prevention and Control of Explosive Disasters, Beijing Institute of Technology,Beijing 100081,China);Thermodynamic study of adsorption of catechin on ion exchange fiber[J];Journal of Chemical Industry and Engineering(China);2005-07
8 ZHU Bin1,SONG Hang2,CHEN Xiaoguang1,LU Tan2,Li Renquan1(1.Sichuan Vocational and Technical College,Suining,Sichuan 629000;2.Department of Pharmaceutical and Biological Engineering,Sichuan University,Chengdu 610065);Study on preparation process of high purity of tea catechins EGCG from commercial tea polyphenols[J];Journal of Huazhong Normal University(Natural Sciences);2008-04
9 LU Li-hong,TAO Jian-wei,CAI Yu,ZHU Pin-pin,WEI Wei (Dept.of Chem.Engineering,Shanghai College of Applied Techniques,Shanghai 200235,China);Microwave-Assisted Extraction and HPLC Determination of Catechins and Caffeine in Tea[J];Physical Testing and Chemical Analysis(Part B:Chemical Analysis);2008-05
10 GONG Zhi-huaa,b,HUANG Tiana,PANG Yue-lana,XIAO Wen-juna,b (a.College of Horticulture and Landscape; b.Tea Key Laboratory of the Ministry of National Education,HNAU,Changsha 410128,China);Separation and purification of EGCg in catechins by HP-20 macroporous resin[J];Journal of Hunan Agricultural University(Natural Sciences);2010-01
China Proceedings of conference Full-text Database 1 Hits
1 Xiaowenjun, Huangtian, Tanjun, Gongzhihua (Tea Key Lab of the Ministry of National Teaching of Hunan Agricultural University, Hunan, ChangSha 410128);Separatian and Purification of EGCG in Catechin by HP-20 Macroporous Resin[A];[C];2008
【Citations】
Chinese Journal Full-text Database 1 Hits
1 (Li Bingding~1;Tang Feng;Tang Jian~2;Ho Chitang~3(~1Dept of Food Science,Nanjing Agric Univ,Nanjing210014,~2Wuxi Institute of Light Industry,~3Rutgers University,USA);DETERMINATION OF THE COMPOSITION OF TEA ANTIOXIDANT BY GAS CHROMATOGRAPHY AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J];Journal of Nanjing Agricultural University;1992-02
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 Zhang Li, Duan Hongjin, Fang Hongiu, Wang Zengsheng·, Liu Zhonghua·(Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050);Analysis on Catechins and Alkaloids in Tea by HPLC[J];JOURNAL OF TEA SCIENCE;1995-02
2 CHEN Hua-cai~(1,2),LU Jin~1,CHEN Xing-dan~2,LIU Hui-jun~1 (1.Chinese Jiliang University,Hangzhou 310018,China;2.State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China);Near infrared spectroscopic model for determinating total catechins in tea polyphenol powder based on radical basis function network[J];Optics and Precision Engineering;2006-01
3 LI Jing,LI Jing-yin,GUO Yu-feng,DUAN Hui-min,WANG Wei-xiao(College of Sciences,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China);Electrocatalytic action of catechin at modified electrode of multi-walled carbon nanotube[J];Journal of Hebei University of Science and Technology;2006-04
4 Li Bingding(Dept of Food Science,Nanjing Agric Univ,Nanjing210014);ISOLATION AND IDENTIFICATION OF THE ANTIOXIDATIVE COMPONENTS IN TEA ANTIOXIDANT[J];Journal of Nanjing Agricultural University;1992-04
5 SONG Hua-Mang, CHEN Bo, CAI Qing-yun, YAO Shou-zhuo (1. Institute of Chemistry and Engineering, Hunan Normai University,Changsha 410081,China; 2. Institute of Chemistry and Engineering,Hunan University,Changsha 410082,China);THE PRINCIPAL COMPOSITION CLUSTER ANALYSIS FOR CATECHINS AND ALKALOIDS IN TEA[J];Natural Product Research and Development;2003-05
6 CHEN Hua-cai~(1,2), LV Jin~3, FENG Chun-hong~4, LU Yong-jun~2, CHEN Xing-dan~2(1. College of Life Sciences of China Jiliang University, Hangzhou, 310018, China;2. State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics,Chinese Academy of Sciences, Changchun 130033, China; 3. College of Metrological Technology & Engineering of China Jiliang University, Hangzhou 310018, China; 4. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China);Feasibility study on quantitative analysis of total catechins in tea polyphenol powder by near-infrared spectroscopy[J];Journal of China Institute of Metrology;2005-01
7 Gao Kejun1,Xia Tao1,Gao Liping2,Liu Yajun1 (1Key Lab of Tea Biochemistry & Biotechnology,Ministry of Education and Ministry of Agriculture,Anhui Agric University,Hefei,230036; 2Department of Biology Science,Anhui Agricultural University,Hefei 230036);Method of Determining the Product by DFR/LAR Enzyme Reaction Rapidly in Tea[J];Chinese Agricultural Science Bulletin;2008-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang Xuemei Gao Sulian (Department of Chemistry Anhui University Hefei 230039);Studies on Optimum Conditions of Extracting Rough Glycyrrhizic Acid from Glycyrrhiza[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1999-01
2 WANG Wei-hua et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Extraction of Tea Polysaccharide of Puer and Mensuration of its Content[J];Journal of Anhui Agricultural Sciences;2006-06
3 ZHAO Su-qin (Editor Department of Qinghai Nationalities Institute,Xining,Qinghai 810007);Determination of Content of Bergenin in Bergenrae pacumbis by RP-HPLG[J];Journal of Anhui Agricultural Sciences;2006-11
4 WU Xiao-chun et al (Bio-engineering Institute of Yichun University,Yichun,Jiangxi 336000);Study on the Extraction of Total Flavonoids from Ainsliaea fragrans Champ.by Ultrasonic Radiation[J];Journal of Anhui Agricultural Sciences;2006-22
5 LIU Jiao,ZHOU Ya qiu,PENG Chao (Postgraduate Student of Grade 2002,AnHui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);Determine the Contents of Ecdysterone in Achyranthes Bidentata[J];Journal of Anhui Traditional Chinese Medical College;2005-03
6 TAN Xiao-hua; ZHANG Ya-li; ZHOU Dian-yuan(Institute for Hepatic Diseases, Beijing Army General Hospital, Beijing 100700, P R. China)(Institute for Digestive Diseases, Nanfang Hospital, The First Mlitary Medical University,Cuangzhou, 510515, P R. China);EGCG induced apoptosis and expression change of bcl-2 protein in gastric and hepatic carcinoma cells[J];CHINESE JOURNAL OF CANCER;2000-07
7 CHEN Yong-bo (The Southern Potato Reseach Centre in Enshi, Hubei 445000) RAO Bin, XIN Lan (Chinese Japanese Joint Venture Hubei Fengjiang Amino Acid Co.Ltd. 445800);The Application Skills in Accq.Tag Method[J];Amino Acids & Biotic Resources;2001-04
8 GAO Xiao-hong, YUAN Hua (Hubei Research Institute of Chemistry , Wuhan 430074, China);Progress in Analysis and Determination of L - theanine[J];Amino Acids & Biotic Resources;2004-02
9 ZHOU Li,LI Bing-chao(College of Biotechnology,Shenyang Agricultural University,Shenyang 110161,China);Study on Extraction of Flavonoids from Aralia continentalis Kitagwa[J];Amino Acids & Biotic Resources;2007-01
10 Liu Qi Teng Wei Li Ruiju (Contral Laboratory. Shanclong Academy of Agrieutural Scienees. Jinen 250100);Studies On Comprehensive process and Quality Analysis of Jujube[J];AMINO ACIDS & BIOTIC RESOURCES;1997-04
China Proceedings of conference Full-text Database 2 Hits
1 Guo Jichun, Ye Naixing, He Xiaoyan, Zhang Wenjin, Yang Ruxing, Chen Zhihui (Tea Research Institute, Fujian Academy of Agricultural Sciences, 355000);Advance on the Research of Oolong Tea Germplasm Resource[A];[C];2000
2 CHEN Liang, YANG Yajun (Tea Research Institute, Chinese Academy of Agricultural Sciences, 310008);The Application of RAPD in the Investigation of Tea Breeding and Germplasm[A];[C];2000
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 SUN Hai-yan et al(Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500);Effects of Tea Polyphenol on the Physiological Characteristics of Cucumber Seedlings under Draught Stress[J];Journal of Anhui Agricultural Sciences;2007-36
2 LI Xin et al (College of Science,Henan Agricultural University,Zhengzhou,Henan 450002);Study on the Adsorption Properties of L-Proline on Ion Exchange Fiber[J];Journal of Anhui Agricultural Sciences;2008-10
3 LIAO Bu-yan,ZHANG Zheng-zhu,XIA Tao,ZHU Bo,YAN Shou-he,WAN Xiao-chun(Key Laboratory of Tea Biochemistry & Biotechnology,Ministry of Education and Ministry of Agriculture,Anhui Agricultural University,Hefei 230036);Application of near infrared spectroscopic techniques on the fidelity identification of tea quality[J];Journal of Anhui Agricultural University;2009-02
4 HAN Liping,XU Hairong,NI Jun(Department of Tea Science,Zhejiang University,Hangzhou 310029);Near-infared spectroscopy technique and its applications in tea research[J];Journal of Tea;2007-01
5 LUO Wenwen, ZHANG Yueling, GONG Shuying, GU Zhilei (Zhejiang University Tea Research Institute, Hangzhou 310029,China);Preliminary construction of NIR calibration model for moisture and total tea polyphenols of green tea[J];Journal of Tea;2007-02
6 LI Ming-yu,ZENG Qing-xuan,FENG Chang-gen,SUN Wei-na,LI Kai(State Key Laboratory of Explosion Science and Technology,Beijing Institute of Technology,Beijing 100081,China);Structure and properties of the strong alkaline anion exchange fiber[J];Materials Science and Technology;2006-01
7 ZHOU Jian, CHENG Hao P* P, WANG Li-yuan (Research Center for Tea Germplasm and Improvement, Tea Research Institute, Chinese Academy of Agriculture Science, Hangzhou 310008, China);Recent Advance on the Application of Near-infrared Spectroscopy in Tea[J];Journal of Tea Science;2008-04
8 ZHENG Hong-fa,HUANG Ya-hui(Hunan Tea Research Institute,Changsha 410125,China);Study and Development of High-content EGCG Tea[J];Tea Communication;2007-01
9 ZENG Hong-hu1,2,ZHANG Ai-li2,TONG Xiao-wei2,JI Rui-lin2,WANG Shuang-fei1,ZHU Yi-nian2,LI Feng2,YU Zheng-hong(1.Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530005,China;2.a.Guangxi Key Laboratory of Environmental Engineering,Protection and Assessment;b.Department of Resources and Environmental Engineering,Guilin University of Technology,Guilin 541004,China);Experiment of the Phosphate Adsorption Characteristics by Peat[J];Journal of Guilin University of Technology;2008-01
10 WEI Dian-wen et al(Nature and Ecology Institute,Heilongjiang Science Academy,Harbin 150040,China);The development of extraction and separation of lignans from Schisandra chinensis(Turcz.) Baill.[J];Territory & Natural Resources Study;2008-01
China Proceedings of conference Full-text Database 1 Hits
1 ZENG Qiu-wen, QIN Xiao-ming , LIN Hua-juan, CHEN Shu-Qing, HUANG Bin-Jie (College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China);Study on Preliminary Isolation and Purification of Saponin from the Leaves of Camellia chrysantha (Hu) Tuyama[A];[C];2009
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved