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《Journal of Tea Science》 2009-02
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Sampling Strategy to Establish a Primary Core Collection of Chinese Tea Germplasms

WANG Xin-chao,LIU Zhen,YAO Ming-zhe,MA Chun-lei, CHEN Liang,YANG Ya-jun (Research Center for Tea Germplasm and Improvement,Tea Research Institute Chinese Academy of Agricultural Sciences;National Center for Tea Improvement,Hangzhou 310008,China)  
In order to rapid and precisely identify the excellent gene resource for the breeding of tea plant,the construction on core collection of tea plant is necessary. Based on the passport and evaluated datum of 1048 tea accessions in "Evaluated database of tea germplasms",the optimum sampling proportion,grouping methods,intra-group sampling proportion and methods,in order to obtain the optimum sampling strategy to establish the core collection of China tea germplasm. Results showed that,(1) 20% was the optimum sampling proportion,at this proportion,the genetic diversity index of core collection was higher than other proportions,and the reserved ratio of phenotypic charater of core collection was higher than 95%.(2) At 20% sampling proportion,25 primary core collections using 3 grouping methods,4 intra-group sampling proportions,2 intra-group individual selecting methods were evaluated using 5 parameters to determine the optimum sampling methods. The five parameters were a) ratio of phenotype reserved,b) phenotypic of variance value,c) variance of phenotypic frequency,d) Shannon-weave genetic diversity index,e) coefficient of variation quantitative characters. Results showed that there were different ranks when different evaluated parameter was used. It implied that there was significant interaction between groupingmethods,sampling proportion and sampling methods within group. Combined the ranking results of 5 parameters,the optimum sampling strategy to establish primary core collection of tea plant is cultivated region grouping-logarithm sampling proportion-cluster individual selecting.
【Fund】: 浙江省自然科学基金项目(Y305124);; 国家科技基础条件平台工作项目“多年生和无性繁殖作物种质资源标准化整理整合共享试点”(2005DKA21002-08)内容之一。
【CateGory Index】: S571.1
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