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Effect of Pu-erh Tea on Bone Mineral Densityin Wistar Rats

HOU Yan1,XIAO Rong2*,SHAO Wan-fang1,ZHOU Ling1,YANG Lan-lan3,GONG Jiang3,YANG Lei1,WANG Guo-cheng1(1.College of Long Run Pu-erh Tea,Yunnan Agricultural University,Kunming 650201,China;2.College of Food Science,Yunnan Agricultural University,Kunming 650201,China;3.College of Animal Science,Yunnan Agricultural University,Kunming 650201,China)  
The effects of Pu-erh tea on the indexes of bone mineral density and bone growth in wistar rats were investigated.100 male wistar rats were divided into 10 groups of 10 rats each randomly.The first group severed as control.The 9 treatments groups were given daily oral administrations(20 mL/kg) of fermented Pu-erh tea,unfermented Pu-erh tea or green tea tract at the conversation listed above,which yielded dose of 0.5,1.0 or 2.0 g/kg(body weight).The control group were given oral administrations of distilled water.After 90 d of treatment,the rats were sacrificed,peeled left femur and the weight of femur,the content of calcium and phosphorus in bone,the density of bones etc were determined.The resulted showed that compared to the control group,the weight of left femur in groups treated with either low,medium dose of unfermented Pu-erh tea and green tea or each dose of fermented Pu-erh tea was not statistically signification(P0.05).The content of calcium in bone in groups treated with either the highest dose of unfermented Pu-erh tea and green tea or low,median dose of fermented Pu-erh tea was significantly higher than the control group(P0.05) while the content of phosphorus in each group was significantly higher than the control group(P0.05).The density of each group of fermented and unfermented Pu-erh tea and green tea was not statistically signification(P0.05).Under the experimental conditions,these tea did not affect bone mineral density of rats.
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