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Studies on the Extraction of Polysaccharide From Middle and Lower Middle Grade Green Tea and the Effectiveness on Blood-glucose Depressing

Li Buqing;Zhang Huiling;Shu Qingling;Zhang Buchang;Ge Shengfang(Institute of Green Food Engineering,Anhui Academy of Agricultural Sciences,Hefei 230031)  
The isolation andpurification of tea polysaccharide were carried out and the blood-glucose pressing activity of the polysaccharide was investigated Sedimentated with CTAB and acetone and deproteined with Sevag method,the polysacccharide could be obtained frorn the cold water extract of the green tea. The polysaccharide was purified with DEAE-Cellulose column chromatography,with 69%glycouronic acid and 4.66%protein.It was proved that the tea polysaccharide was an acidic glycoprotein.Pharmacologic experiment revealed that acetone-sedimentated polysaccharide showed stronger blood-glucose depressing activity than that treated with TCA or ethanol.The blood-glucose of the mice dropped to 30%of the control level after 7 hours of injecting the mice with 3 00mg/kg acetonesedimentated polysaccharide.Experimentshowed that the polysaccharide could decrease hyperglycemia caused by alloxan or ephephdne,and theeffectiveness was similar to that of insulin.
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