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《Pratacultural Science》 2005-10
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RAPD Analysis of Elymus dahuricus,Hordeum brevisubulatum,Their Hybrid F_1 and BC_1

LI Zao-zhe~1,YUN Jin-feng~1,YIN Jun~2(1.College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Hohhot 010019,China;2.College of Bio-engineering,Inner Mongolia Agricultural University,Hohhot 010019,China)  
The genetic differences among Elymus dahuricus,Hordeum brevisubulatum,their hybrid F_1 and BC_1 were investigated by using RAPD technique.The results showed that the genomic DNA in tested materials had plentiful polymorphisms;the genetic differences between E.dahuricus and H.brevisubulatum were relatively great,both E.dahuricus×H.brevisubulatum F_1 and H.brevisubulatum×E.dahuricus F_1 tended to inherit toward E.dahuricus,the genetic differences between E.dahuricus×H.brevisubulatum F_1 and H.brevisubulatum×E.dahuricus F_1 were not significant,BC_1 tended to inherit toward recurrent parent H.brevisubulatum,there were differences in gene among BC_1 lines;RAPD technique can be used to identify hybrid and to detect the plants with object traits.
【Fund】: 国家自然科学基金资助(30260074)
【CateGory Index】: S543.9
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