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《Pratacultural Science》 2007-03
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Genetic transformation of DREB2A gene into alfalfa

SHENG Hui,ZHU Yan-ming,LI Jie,BAI Xi,CAI Hua (Northeast Agriculture University,Harbin 150030,China)  
With "ZhaoDong"、"AoHan"、"No.1 of GongNong"、"No.2 of GongNong"as researching material,the concentration and sorts of antibiotics and kanamycin sensitivity were systematically discussed,and high efficiency regeneration and genetic transformation system of alfalfa was established; constructed two plant expression vectors with DREB2A gene regulated by inducible expression promoter rd29A and constitutive expression promoter E12.Genetically transformed into alfalfa by way of Agrobacterium Tumefaciens and acquired a lot of transgenic plants and identified by molecule biology detection(PCR and Southern blot).The result showed that,DREB2A gene had been joined into genome of alfalfa.
【Fund】: 黑龙江省科技厅重点攻关项目(GB05B104);; 中国博士后科学基金
【CateGory Index】: S541.9
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【References】
Chinese Journal Full-text Database 4 Hits
1 HUANG Ying-xin1,2,ZHOU Dao-wei3,YUE Xiu-quan3,YANG Ji-yun3(1.Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou 730000,China;2.School Graduates,Chinese Academy of Science,Beijing 100049,China;3.Institute of Grassland Science,Northeast Normal University,Key Laboratory for Vegetation Ecology,Ministry of Education,Changchun 130024);Research progress of Medicago ruthenica[J];Pratacultural Science;2007-12
2 FENG Li-xing1,FU Li1,LIU Wei2,SUN Jie2,CHEN Ai-min3,WANG Yan-zhang3(1.College of Food Science,Xinjiang Agricultural University,Urumqi 830000,China;2.College of Agronomy,Shihezi University,The Key Oasis Eco-agriculture Laboratory of Xinjiang Bingtuan,Shihezi,Xinjiang 832000,China;3.Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China);Establishment of high frequency regeneration system of Medicago truncatula with cotyledonary-node explants[J];Pratacultural Science;2008-08
3 CAI Hua,ZHU Yan-ming,BAI Xi,LI Yong,JI Wei(College of Life Science,Northeast Agricultural University,Harbin 150030,China);Cloning and analysis of a novel DREB gene of Glycine soja[J];Pratacultural Science;2009-08
4 HUANG Xin~1 YE Hong-xia~2 SHU Xiao-li~(2) WU Dian-xing~2(1.Institute of Animal Sciences and Veterinary Medicine,Zhejiang Academy of Agriculture Sciences,Hangzhou,Zhejiang 310021;2.IAEA Collaborating Center/Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou,Zhejiang 310029);ADVANCES IN IMPROVEMENT OF STRESS TOLERANCE BY INDUCED MUTATION AND GENETIC TRANSFORMATION IN ALFALFA[J];Journal of Nuclear Agricultural Sciences;2008-05
【Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Jie,ZHU Yan-ming,WU Di,YANG Ai-fu,BAI Xi( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Cloning and sequencing of DREB2A gene and construction of its plant expression vector[J];Journal of Northeast Agricultural University;2005-01
2 JI Wei,LI Jie,ZHU Yan-ming,BAI Xi,DAI Cui-hong,HOU Ai-ju ( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Transfermation of DREB1A gene regulated by different promoters into cucumber[J];Journal of Northeast Agricultural University;2005-04
3 WAN Dongshi1,LI Hongyu1*,ZHANG Lixin1,2,LIANG Houguo1(1 Department of Biochemistry and Molecular Biology,Life School,Lanzhou University,Lanzhou 730000,China;2 Shapotou Desert Experiment and Research Station of Cold and Arid Regions Envioronmental and Engineering Institute,Chinese Academy of Sciences,Lanzhou 730000,China);Gene expression and signal transduction of plants under drought stress in vivo[J];Acta Botanica Boreali-occidentalia Sinica;2003-01
4 WANG Shao-Xia, WANG Zhen-Ying*, PENG Yong-KangDepartment of Biology, Tianjin Normal University, Tianjin 300074;Dehydration Responsive Element Binding (DREB) Transcription Activator and Its Function in Plant Tolerance to Environmental Stresses[J];Plant Physiology Communications;2004-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GE Bao-yu LIN Yi HOU He-sheng (College of life science Liaoning Normal University,Liaoning Dalian 116029);Structure and Function of ERF Transcription Factors[J];Anhui Agricultural Science Bulletin;2007-20
2 Chen Xia Luo Shiqiao Duan Cuifang Zeng Rizhong(Rubber Research Institute /Ministry of Agriculture Key Lab for Tropical Crops Physiology,CATAS,Danzhou,Hainan,571737,P.R.China);Research Advance on the Transcription Factors in Higher Plants[J];Anhui Agricultural Science Bulletin;2008-09
3 Chen Zhe et al.(Department of Life Science,Normal University,Kunming 650092,China);Research Progress of Gene Engineering in Plant Stress Tolerance[J];Anhui Agricultural Science Bulletin;2009-07
4 LIU Ke-jie et al (Shenyang Agricultural University, Shenyang,Liaoning 110161);Construction of Plant Transformation Vector of FaDREB1 Gene for RNAi[J];Journal of Anhui Agricultural Sciences;2005-07
5 LI Xin-ling et al (Teaching and Research Section of Forestry Genetics and Breeding,Northeast Forestry University,Harbin,Heilongjiang 150040);Cloning and Construction of Expression Vector of Promoter rd29A and CBF2 Gene Related to Adversity Stress[J];Journal of Anhui Agricultural Sciences;2007-13
6 WANG Tian-xiang et al (College of Biological Sciences and Biotechnology of Beijing Forestry University,Beijing 100083);Gene Cloning of AtDREB2B and Constructtion of Its Plant Expression Vectors[J];Journal of Anhui Agricultural Sciences;2008-21
7 LIU Wen-ping1,LU Shu-wen1,LIU Jian-xin1,NAN Xiang-ri1,LIU Jing-lan2,LI Zhu-gang1(1.Biotechnological Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086,China;2.Horticultural Sub-academy,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150069,China);Agrobacterium-mediated Transformation of Cucumber for BnCS Gene[J];Northern Horticulture;2009-01
8 QI Chun-hui;HAN Lie-bao;LIANG Xiao-hong;ZENG Hui-ming;LIU Jun.Key Laboratory for Silviculture and Conservation,Ministry of Education,Institute of Turfgrass Science,Beijing Forestry University,100083,P.R.China.;Transgenic Zoysia japonica plants obtained by biolistic bombardment transformation.[J];Journal of Beijing Forestry University;2006-03
9 SHAO Hong-bo~(1,2,3),LIANG Zong-suo~(2,3,4),SHAO Ming-an~(2,5)(1.Biological Science Laboratory,College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;2.the State Key Lab of Soil Erosion and Dryland Farming on the Loess Plateau,the Center of Soil and Water Conservation and Eco-environmental Research of the Chinese Academy of Sciences,Yangling 712100,China;3.Molecular Biology Laboratory,Bioinformatics College,Chongqing University of Posts &Telecommunications,Chongqing 400065,China;4.Life Science College,Northwest Sci-tech University of Agriculture and Forestry,Yangling 712100,China;5.Institute of Geographic Sciences and Resource,the Chinese Academy of Sciences,Beijing 100101,China);Progress and trends in the study of anti-drought physiology and biochemistry,and molecular biology of Triticum aestivum[J];Acta Prataculturae Sinica;2006-03
10 JI Wei,LI Jie,ZHU Yan-ming,BAI Xi,DAI Cui-hong,HOU Ai-ju ( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Transfermation of DREB1A gene regulated by different promoters into cucumber[J];Journal of Northeast Agricultural University;2005-04
China Proceedings of conference Full-text Database 4 Hits
1 HAO Xiao-yan~(1,2) CHEN Ming~2 XU Hui-jun~2 GAO Shi-qing~2 CHEN Xian-guo~3 LI Lian-cheng~2 DU Li-pu~2 YE Xin-guo~2 MA Youzhi~(2*) 1.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China; 2.National Key Science Facility of Crop Gene Resources and Gene Improvement,Key Laboratory of Crop Genetic and Breeding,Ministry of Agriculture,Institute of Crop Science,Chinese Academy of Agricutural Sciences,Beijing 100081; 3.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricutural Sciences,Beijing 100081,China;Obtaining of transgenic wheats with GH-DREB gene and their Physiological index analysis on drought Tolerance[A];[C];2005
2 WANG Qiao-yan~(1,2) CHEN Ming~1 QIU Zhi-gang~1 CHEN Xian-guo~3 XU Zhao-shi~1 LI Lian-cheng~1 MA You-zhi~(1*) 1.National Key Science Facility of Crop Gene Resources and Gene Improvement,Key Laboratory of Crop Genetic and Breeding,Ministry of Agriculture,Institute of Crop Science,Chinese Academy of Agricutural Sciences,Beijing 100081,China; 2.College of Agronomy,Xinjiang Agricultural University,Xinjiang Urumqi 830052,China; 3.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agrieutural Sciences,Beijing 100081,China;Cloning and identification of a new gene encoding DREB transcription factors from Glycine max L.[A];[C];2005
3 LI Wei-ming, ZHANG Han-shuang, ZHAO Jun-li, FU Shu-ping(Cotton Institute,Hebei Academy of Agriculture and Forestry Science,Shijiazhuang 050051, China);Advances in Gene Engineering on Plant Salt-tolerance and Key Research Aspects of Cotton Salt-tolerance in the Future[A];[C];2005
4 XU Chun-bo~1,WANG Yong~2,LI Xing-you~1,ZHAO Hai-xia~1 (1.Grassland Research Institute,Chinese Academy of Agricultural Sciences,Huhhot 010010,China; 2.College of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot 010018,China);Study on establishment and optimization of regeneration system of Medicago sativa L.cv. Gongnong No.1[A];[C];2009
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ke-wei 1, WANG Xian-yan 1, WANG Ling-zhi 1, ZHANG JU-ren 1, XU Fang-zuo 2, XU Li-hua 2, ZHOU Zhu-hua 2 (1 School of Life Science, Shandong University, Jinan 250100,China; 2 Institute for application of Atomic Energy,Shandong Academy of A;Breeding of maize inbred lines and hybrids with the trait of salt-tolerance via cellular engineering[J];Shandong Agricultural Sciences;2001-06
2 LI Hong-yu et al(College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161);High Efficient System of Plant Regeneration for Genetic Transformation of Lilium[J];Journal of Anhui Agricultural Sciences;2005-12
3 QIAO Yong-xu et al (Department of Life Science,Tangshan Teacher's College,Tangshan,Hebei 063000);Regeneration of Lilium oriental hybrid of Sorbonne[J];Journal of Anhui Agricultural Sciences;2007-01
4 ZHOU Yan-ping;ZHENG Hong-juan;JIA Gui-xia.Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,National Floriculture Engineering Research Center,Beijing Forestry University,100083,P.R.China.;Establishment of regeneration system on two species of Lilium asiatic hybrid in vitro.[J];Journal of Beijing Forestry University;2007-01
5 Qi Qiuhui (Institute of Botany, Academia Sinica, Beijing 100044);Studies on Properties of Photsynthetic Ecology in Melilotides ruthenica[J];Acta Agrestia Sinica;1996-02
6 Du Zhanchi(Commission for Integrated Survey of Natural Resources, Academia Sinica, Beijing 100101)Yang Zonggui(Institute of Botany, Academia Sinica, Beijing 100093);Dynamic Characteristics on the Relationships Between Net Photosynthetic Rate and Light Intensity in Shoots of Melissitus ruthenica,Artemisla frigida and Kochia prostrata[J];ACTA AGRESTIA SINICA;1997-03
7 Luo Xinyi; Li Hong(Animal Science Institute of Heilongjiang, Fularji 161041);The Yield Stability of the Hybrids Between Pocockia ruthenica and Medicago sativa[J];ACTA AGRESTIA SINICA;1997-03
8 Jin Hong ;Li Gao; Wu Yunfei(Department of Grassland Science, Inner Mongolia Institute of Agriculture and Animal Husbandry, Huhhot 010018);Studies on the Histology of Organogenesis in Isolated Culture of Mature Embryo of Melilotoides ruthenicus(L.) Sojak cv.‘Zhili'[J];ACTA AGRESTIA SINICA;1997-04
9 LI Bo, JIA Xiu-feng, GAO Mei-ling, BEN Wen-wei (College of Life Science and Engineering, Qiqihar University , Qiqihar,Heilongjiang Province 161006, China);A Research on the Cold-Resistance of Induced Alfalfa Calluses[J];Acta Agrestia Sinica;2004-02
10 LIU Yan-zhi, WANG Yu-min, LIU Li, SUI Song-bing, LI Wang-feng, DONG Ying-shan~*(Biotechnology Research Center, Jilin Academy of Agri culture Sciences, Gongzhuling, Jilin Province, 136100, China);Agrobacterium-Mediated Transformation of Alfalfa Variety,Caoyuan No.1,by Bar Gene[J];Acta Agrestia Sinica;2004-04
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 MAO Ya-ni1,SUN Juan2,ZHANG De-gang1,SHENG Yi-bing3,YANG Guo-feng2(1.College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China;2.Qingdao Agricultural University,Qingdao 266109,China;3.Shandong Institute of Agricultural Sustainable Development,Jinan 250100,China);Research progress in Medicago spp. tissue culture[J];Pratacultural Science;2009-09
2 GUO Jian-kang,ZHAO Min-jie,TAO Ji,FENG Jin-chao(College of Life and Environmental Sciences,Central University for Nationalities,Beijing 100081,China;Institute of Resource and Environment Protection for Minority Area of China;Beijing 100081,China);Plant Diversity of Desert Vegetation in Naiman Region,Inner Mongolia[J];Journal of the Central University for Nationalities(Natural Sciences Edition);2008-S1
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 LI Jie,LI Jing,ZHU Yan ming,ZHANG Jing, DAI Cui hong,JI Wei,CAI Hua (Northeast Agricultural University,Harbin,Heilongjian 150030,PRC);Construction of plant transformation vector of DREB1A gene[J];Journal of Northeast Agricultural University;2003-02
2 LU Ping 1,LU Qing 2,DU Rong qian 2 (1. Department of Biology,Inner Monglia Normal University,Huhhot 0l0022,China; 2. College of Life Science,Nankai UniverSity,Tianjin 300071,China);THE ADVANCE IN LEA GENE AND LEA PROTEIN RESEARCH[J];JOURNAL OF INNER MONGOLIA NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1999-02
3 Huang Rongfeng1 Yang Yuhong2 Wang Xuechen3 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081; 2 Vegetable Institute, Chinese Academy of Agricultural Sciences, Beijing 100081; 3 College of Biological Sciences, C;Molecular Mechanism of Plant Responses to Low Temperature[J];Journal of Agricultural Biotechnology;2001-01
4 Ph.D & Associate Professor ZHANG Zhengbin Member of CEA SHAN Lun (Institute of Soil and Water Conservation,Chinese Academy of Science and Ministry of Water Resources, Yangling 712100);Advance in Drought Resistance Transgenic Crop[J];WORLD SCI-TECH R & D;1999-02
5 Hou Aiju,Zhu Yanming,Yang Aifu,and Zhang Binbin (Plant Bioengineering Laboratory,Life Science Institute,North East Agriculture University,Harbin 150030,China);Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro[J];Acta Horticulturae Sinica;2003-01
6 ZHANG Lin-Sheng, ZHAO Wen-Ming ( The School of Life Science and Technology, Xi'an Jiaotong University 710049; College of Life Science, Northwest Science & Technical University of Agricultural and Forestry, Yangling, Shaanxi 712100);LEA Protein Functions to Tolerance Drought of the Plant[J];Plant Physiology Communications;2003-01
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