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《Pratacultural Science》 2007-05
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Study of seed protein band pattern diversity of 40 wild populations of Lespedeza

YAN Wei-hong1,XU Zhu2,SHI Wen-gui2,LI Lin-hang2,MA Yu-bao2(1.Chinese Academy of Agriculture Sciences,Beijing 100081,China;2.Grasland Research Institute of Chinese Academy of Agriculture Science,Inner Mongolia,Huhhot 010010,China)  
Genetic diversity of seed storage protein was estimated on 40 wild Lespedeza populations(3 species and 2 varied species) from Inner Mongolia,Hebei,Shanxi,Ningxia and Gansu provinces,using SDS-PAGE techniques.Total 32 bands were found varing in molecular weight from10.3KDa to 108.1KDa;among which,4 bands were shared by all the populations and other 28 showed(90.6%).The genetic similarity coefficient varied from 0.42 to 1.00,giving greater genetic variation and higher polymorphism.Clustering with UPGMA using genetic similarity coefficient at 0.581 showed the existence of 4 different groups.Three populations of L.bicolor were classified as the first group.The second group consisted of 23 populations,including 11 populations of L.potaninii and 12 populations of L.davurica.Two populations of L.potaninii were included in the third group.The fourth group was made up of 4 populations of L.hedysaroides and 8 populations of L.hedysaroides.var.subsericea.At species level,a majority of populations from the same or similar areas were gathered together.L.davurica was genetically close to L.potaninii;while L.hedysaroides was close to L.hedysaroides var.subsericea,indicating that cluster analysis coincided with the results of morphologic identification. Therefore,abundant protein diversity among Lespedeza species could be an important reference to intraspecies variation and ecological type research.
【Key Words】： Lespedeza seed protein diversity wild population
【Fund】： 国家重点基础研究发展计划(973)生态系统服务功能与生态-生产功能区优化布局(G2007CB106806)
【CateGory Index】： S541.5
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