Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pratacultural Science》 2009-01
Add to Favorite Get Latest Update

Study on germination conditions of hybrid embryo of Caucasian clover × white clover

YANG Zhen,HE Li-jun,WANG Ming-jiu,MIAO Qing-wang,CHEN Hai-jun (College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Huhhot 010019,China)  
Sixploid Caucasian clover and tetraploid white clover were used as distant hybridization parents to test the germination conditions of hybrid embryo by using tissue culture.The results showed that the highest germination rate(8%)could be obtained under the conditions of Caucasian clover as the female parent,white clove as the male parent,pollinated at 8:00 to 9:00,the embryo was picked after 12 to 13 days from pollination,the hybrid embryo was sterilized by NaClO(10%)for 6.5 to 7 min,cultured on eight improved MS media.For higher frequency propagation,the germination was higher on the above mentioned medium added with 0.25 mg/L of 2,4-D and 0.50 mg/L of 6-BA.
【Fund】: 国家自然科学基金(30660118);; 内蒙古教育厅基金(NJC07051)
【CateGory Index】: S541.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 LUAN Xiao-min1,WANG Ming-jiu1*,HE Li-jun2,LI-Yao1,LI Zhi-qiang1(1.College of Ecology and Environmental Science,Inner Mongolia Agriculture University,Huhhot,Inner Mongolia Autonomous Region 010019,China;2.College of Agronomy,Inner Mongolia Agriculture University,Huhhot,Inner Mongolia Autonomous Region 010019,China);Screening of Embryogenic Callus from Hybrid F1 of Caucasian Clover×White Clover[J];Acta Agrestia Sinica;2009-05
2 HUANG Fan1,WANG Ming-jiu1,HE Li-jun2,CHEN Li-li1(1.College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Inner Mongolia Huhhot 010019,China;2.College of agronomy,Inner Mongolia Agricultural University,Inner Mongolia Huhhot 010019,China);Study on organogenesis of stem segments in vitro of F1 generation of Trifolium ambiguum×T. repens[J];Pratacultural Science;2010-05
3 LI Run-fang,HUI Rong-kui,DENG Rui-ning,CAI Ming-li,LIU Yan(College of Plant Science and Technology,Huazhong Agricultural University,Hubei Wuhan 430070,China);Genetic diversity of clover by SRAP[J];Pratacultural Science;2010-12
4 ZHANG Jing-yuan,PENG Yan,LUO Yan,MA Xiao (Department of Grassland,Sichuan Agricultural University,Ya’an 625014,China);Genetic diversity in white clover germplasm detected by SRAP markers[J];Acta Prataculturae Sinica;2010-05
Chinese Journal Full-text Database 10 Hits
1 WU Wei-qun~1,TANG Zong-ying~2,DENG Ju-fen~1,ZHANG Ying-jun~3,HUANG Bi-zhi~4,GENG Wen-cheng~5 (1.Yunnan Provincial Pasture and Fodder Station,Kunming, Yunnan Province 650225,China;2.Yunnan Agriculture University,Kunming,Yunnan Province 650201,China;3.Institute of Grassland Science,China Agriculture University,Beijing 100094,China;4.Yunnan Beef Cattle and Pasture Research Centre.Kunming,Yunnan Province 650212,China;5.Yunnan Provencial Sheep Farm,Kunming,Yunnan Province 655204,China);Effect of Row Spacing and Sowing Rate on the Growth and Seed Yield of White Clover[J];Acta Agrestia Sinica;2006-02
2 LI Hai-xian~(),SHI Feng-ling (College of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot 010019,China);Current situation of yield improvement and seed production of Melilotoides ruthenica in China[J];Grassland and Turf;2006-03
3 LI Zhi hua,SHEN Yi xin,NI Jian hua,ZHAO Ling ling (College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095,China);A preliminary study on the allelopathy of legumes[J];Pratacultural Science;2002-08
4 YAN Min1,ZHANG Ying-jun1,LI Wei-hong1,HU Han-li2(1.Institute of grassland science,China Agricultural University,Beijing 100094,China;2.Yunnan Grassland and Feed Management Station,Kun Ming 650225,China);Effects of water on nutrition and hormone in white clover stem and leaf before and after anthesis[J];Pratacultural Science;2007-05
5 JIA Wen-qing1,LIU Hui-chao1,HE Li2(1.School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,453003,China;2.Zhoukou Vocational Technical College,Zhoukou 466000,China);Seed germination characteristics of Trifolium repen under salt stress[J];Pratacultural Science;2007-09
6 HAN Jian-Qiu1,ZHANG Zhi-guo2 (1.Horticulture science and engineering college of Shandong agricultural university,Tai'an 271018,China;2.Resource and environment college of Shandong agricultural university,Tai'an 271018,China);Effects of water stress on dry matter allocation and root growth of white clover[J];Pratacultural Science;2007-11
7 LIU Jin-ping,WU Fa-hong,WANG Dan,LIU Yang-jing,ZHU Tao(Agronomy College,South China University of Tropical Agriculture,Danzhou 571737,China);Callus culture and plant regeneration of Stylosanthes guianensis[J];Acta Prataculturae Sinica;2007-03
8 Wang Falin 1 ,Zhao Xiumei 2 ,Li Hongxu 1 ,and Hao Yan 1 ( 1 Pomology Institute of Gansu Academy of Agricultural Sciences,2 Biotechnology Center of Gansu Academy Sciences,Lanzhou,Gansu730070Chi na);Study on Culture in vitro of Interspecies Hybrid Embryo Between Plum and Apricot[J];Journal of Fruit Science;2003-02
9 XU Jian-lan,MA Rui-juan,DU Ping,SHEN Zhi-jun,YU Ming-liang (Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Technique of Embryo Culture in Early-ripening Peach[J];Jiangsu Journal of Agricultural Sciences;2005-04
10 LIU Jin-yu,CAO Li-ping (Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China);Effect of Pollination Time on Squash Seed Quantity and Quality[J];Journal of Shanxi Agricultural Sciences;2006-02
Chinese Journal Full-text Database 10 Hits
1 WANG Jing-min1,LIU Yu-dong2,LI Jing-hua1,LIU Chao1,LI Bing-hua1(1.Zibo Academy of Agricultural Sciences,Zibo 255033,China; 2.Zibo Science and Technology Information Research Institute,Zibo 255000,China);Study on High-yielding and High-quality Seed Production Techniques for Zucchini[J];Shandong Agricultural Sciences;2012-03
2 LI Fan(College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030);Effect of Culture Condition on Pollen Germination of Weigela florida[J];Journal of Anhui Agricultural Sciences;2007-11
3 LIN Mao et al (Guizhou Institute of Oil Crops,Guiyang Experimental Station of National Modern Industry Technology Building system for Rape,Guiyang,Guizhou 550006);The Influence of Continued Congelation on Rape in Guizhou in 2008[J];Journal of Anhui Agricultural Sciences;2008-22
4 LI Rui-hua et al(Grassland College of Gansu Agricultural University,Grassland Ecosystem Key Laboratory of Education Ministry,Research Center on Sustainable Development of China-America Grassland Animal Husbandry,Lanzhou,Gansu 730070);Allelopathy of Root Exudates of Onobrychis viciaefolia on 7 Kinds of Weed[J];Journal of Anhui Agricultural Sciences;2009-15
5 ZOU Ya-li et al(Biochemical Schoo of Tianshui Normal College,Tianshui,Gansu 741001);Effect of the Extract from the Soil around the Alfalfa Roots on Three Species of Plant Seed Germination and Seedling Growth[J];Journal of Anhui Agricultural Sciences;2009-26
6 LUO Chang-min et al (College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Effects of Chemical Hybridizing Agent SX-1 on Brassica napus L.in Chongqing Province[J];Journal of Anhui Agricultural Sciences;2010-13
7 LI Gui-rong et al(Department of Horticulture,Henan Insititute of Science and Technology,Xinxiang,Henan 453003);The Influence of Boric Acid and Sucrose on Vitality of Lily Pollen[J];Journal of Anhui Agricultural Sciences;2010-18
8 ZHAO Hong-yong1,ZHAO Feng2 (1 Baimashi Town Government,Lianshan Huludao Liaoning 125001 China; 2 Liaoning Institute of Pomology,Xiongyue,Liaoning 115009 China);Reviews of the Apricot Germplasm Resources and Genetic Breeding in China[J];Northern Fruits;2008-02
9 QIN Zhi-hua,SUN Yu-gang,YAN Gui-hong,WEI Guo-qin,LI Fang-dong(Shandong Institute of Pomology,Taian,Shandong 271000);Embryo Culture and Proliferation of Interspecies Hybrids of Sweet Cherry[J];Northern Horticulture;2010-08
10 YANG Li,SUN Hao-yuan,ZHANG Jun-huan,WANG Yu-zhu(Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093);Brief Introduction of Research Progress on the Breeding of Apricot in China[J];Northern Horticulture;2011-12
China Proceedings of conference Full-text Database 2 Hits
1 YANG Zhen HE Li-jun WANG Ming-jiu (Inner Mongolia Agricultural University,Inner Mongolia,Huhhot 010019,China);Study on germination conditions of hybrid embryo of Caucasian clover×white clover[A];[C];2007
2 Meng Xin Chen Jinyong Zhao Shiwei (Beijing Botanical Garden,Beijing 100093);Study on Embyo Culture of Syringa vulgaris 'Albo- plena'[A];[C];2011
Chinese Journal Full-text Database 10 Hits
1 LI Kai1,YANG Jian-ping1,MA Ning1,YIN Zhao-ping1,FENG Qi-lun2(1.College of Horticulture Science and Engineering,Shandong Agricultural University/ State Key Laboratory of Crop Biology,Taian 271018,China;2.Shandong Wailian Agricultural Products Processing Co.,Ltd.,Ningyang 271400,China);Study on Cold Resistance of Cucurbita moschata Seedlings under Low Temperature[J];Shandong Agricultural Sciences;2009-01
2 Kong Zheng1 et al. (1 School of Agronomy,Anhui Agricultural University,Hefei 230036,China);Effects of Different Sulfuric Acid Treatment on Seed Germination of White Clover[J];Anhui Agricultural Science Bulletin;2010-23
3 CHEN Xue-fu et al (College of Petrochemical Technology,Lanzhou University of Technology,Lanzhou,Gansu 730050);Research Advances in the Plant of Trifolium L.[J];Journal of Anhui Agricultural Sciences;2006-13
4 XU Xin-xin et al(Department of Gramineous Science,Agricultural and Husbandry College,Qinghai University,Xining,Qinghai 810003);Dynamic Analysis of Alfalfa Grass Yield and Crude Protein Nutrition in Different Harvest Period[J];Journal of Anhui Agricultural Sciences;2007-18
5 LI Xiao-lei et al(Agronomy College,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019);Studies on the Tissue Culture and Regeneration System of Hybrid F_1 of Elymus canadensis×E. excelsus[J];Journal of Anhui Agricultural Sciences;2008-06
6 JIN Zhong-min,SHA Wei(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006);Study on Drought Resistance of Trifolium repens Linn Seedlings[J];Northern Horticulture;2010-18
7 GAO Jing-cao1,WANG Hui-xia2,LI Xi-xuan1(1.Horticultural College of Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100;2.Agricultural Law Enforcement Detachment of Tongchuan City,Tongchuan,Shaanxi 727031);Relationship between Soluble Protein,MDA,and Jujube Tree Cold Hardiness[J];Northern Horticulture;2010-23
8 HUANG Shengyang TU Pengfei (Department of Natural Medicines, Peking University School of Pharmaceutical Sciences; Peking University Modern Research Center for Traditional Chinese Medicine ,Beijing, 100083);Isolation and Identification of Isoflavones from Trifolium pratense[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2004-04
9 YANG Qi-jian~1, ZHOU He~2, SUN Yan~2, LU Ping~1 (~1 Beijing Agricultural College, Beijing 102206;~2China Agricultural University, Beijing 100094 );Study on Induction Callus of Alfalfa and Its Tissue Culture[J];Journal of Beijing Agricultural College;2004-02
10 Li Zaozhe Yun Jinfeng Ma Qingzhi Yu Zhuo (Inner Mongolia Agricultural University,Huhhot 010019);Analysis of Esterase Isozymes of Elymus dahuricus,Hordeum brevisubulatum Their Hybrid F 1 and BC 1[J];ACTA AGRESTIA SINICA;2000-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 DONG Ran1,PAN Yan-yan1,WANG Li-qing2,GU De-feng1,LIU Hong-zhang1(1.Jilin Agricultural University,College of Horticulture,Changchun 130118,China;2.Panshi Horticultural Specialty Technical Popularization Station of Jilin Province,Panshi 132300,China);Establishment of micropropagation system of Ligularia jaluensis Kom.[J];Acta Agrestia Sinica;2010-02
2 LV Zong-you,ZHAO Guo-qi,SU Yan-jing,WU Cai-xia,LU Yin,WANG Xiao-shan(College of Animal Science and Technology,Yangzhou University,Jiangsu Yangzhou 225009,China);A study on leaf protein extraction and purification of white clover[J];Pratacultural Science;2011-11
3 JING Zhao-bin1,YU Jing2,WEI Lin2,CHENG Ji-min1,2,3(1.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,China; 2.College of Resources and Environment,Northwest A & F University,Yangling 712100,China; 3.Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling 712100,China);Establishment of the SRAP-PCR reaction system and selection of the primers in Stipa bungeana[J];Pratacultural Science;2012-02
4 REN Xiao-wei1,2,WANG Yu2,YUAN Qing-hua2(1.College of Pastoral Agricultural Science and Technology,Lanzhou University,Lanzhou 730020,China; 2.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100193,China);Optimization of SRAP-PCR system on Poa pratensis using orthogonal design and selection of primers[J];Pratacultural Science;2012-03
5 YUAN Xue-jun1,2,WANG Zhi-yong1,3,ZHENG Yi-qi1,LIU Jian-xiu1,SHE Jian-ming1(1.Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing 210014,China; 2.College of Life Science,Qiongzhou University,Sanya 572000,China;3.College of Agronomy,Hainan University,Danzhou 571737,China);Acquisition and identification of cold-resistant somatic mutants of centipedegrass[J];Acta Prataculturae Sinica;2011-06
6 LIU Ying1,WANG Xian-gou2,ZHANG Ju-ming1,LIU Fang3(1.College of Agriculture,South China Agriculture University,Guangzhou 510642,China;2.College of Animal Science and Technology,China Agricultural University,Beijing 10093,China; 3.National Center for Animal Husbandry,Beijing 100193,China);Optimization of SRAP-PCR system on Vicia amoena using orthogonal design and selection of primers[J];Acta Prataculturae Sinica;2012-05
7 MA Xiu-jie1,WANG Guan-ming2,HAN Lie-bao1,3(1.Turfgrass Institute of Beijing Forestry University, Province and Ministry Laboratory of Silviculture and Conservation,Beijing 100083,China; 2.Hongming Holdings Company Limited,Beijing 100020,China; 3.College of Gardening and Horticulture of Yangze University,Jingzhou 434025,China);Genetic diversity in Lilyturf germplasm detected by SRAP markers[J];Pratacultural Science;2012-11
8 LI Zhou,PENG Yan,ZHANG Jing-yuan,MA Xiao(College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China);Study on the relationship between morphological variation and geographic origin for white clover[J];Pratacultural Science;2012-11
9 LI Jin-bo;LI Shi-gang;XU Yi-yan;WU Yan-min;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences;College of Pastoral Agri-culture Science and Technology,Lanzhou University;Shenzhen Techand Landscape Co.,Ltd;;Research progress of genetic engineering and stress physiology in white clover[J];Pratacultural Science;2013-11
10 MENG Li-juan;ZHAO Gui-qin;College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability;;Establishment and optimization of ISSR-PCR amplification system for red clover[J];Grassland and Turf;2015-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Jinping 1 ,Cao Ziyi 2 ,Li Wei 2 ,Zhang Jinwen 2 ,Wang Faling 3 (1Agronomy College,South China Uuniversity of Agriculture,Danzhou571737,China;2Agronomy College,Gansu University of Agriculture,Lanzhou730070,China;3Gansu Academy of Agriculture Science,Fruit Research Institute,Lanzhou730070,China);Micropropagation of 5 Almond Cultivars Introduced from U.S.A[J];Northern Fruits;2003-06
2 Qi Qiuhui (Institute of Botany, Academia Sinica, Beijing 100044);Studies on Properties of Photsynthetic Ecology in Melilotides ruthenica[J];Acta Agrestia Sinica;1996-02
3 Jin Hong ;Li Gao; Wu Yunfei(Department of Grassland Science, Inner Mongolia Institute of Agriculture and Animal Husbandry, Huhhot 010018);Studies on the Histology of Organogenesis in Isolated Culture of Mature Embryo of Melilotoides ruthenicus(L.) Sojak cv.‘Zhili'[J];ACTA AGRESTIA SINICA;1997-04
4 Jiang Changshun Huang Huide Zou DongmeiChen Shoucai; Wu Hao(Tropical Field Crops and Animal Husbandry Researeh Institute,Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737);Studies on Tissue Culture of Stylosanthes Guianensis Reyan Ⅱ[J];ACTA AGRESTIA SINICA;1998-01
5 Fang Lining Han Jianguo Wang Pei Liu Yujie (Institute of Grassland Science,China Agricultural University,Beijing 100094) Qu Hongfu Liu Fangqing (Dalian Hongfu Group,Dalian 116001);The Effects of Fertilizer and PGRs on the Seed Yield and Yield Components of Tall Fescue[J];ACTA AGRESTIA SINICA;2000-03
6 YAN Min~1, ZHANG Ying-jun~(1*), HAN Jian-guo~1, GENG Wen-cheng~2, YANG Shi-lin~3 (1.Institute of Grassland Science, China Agricultural University, Beijing 100094, China; 2. Yunnan Stud-stock Farm, Xundian County, Yunnan Province 655205, China; 3. Grassland and Feed Management Section, Kunming , Yunnan Province, 650225, China);Effect of Water Supply on the Growth and Seed Yield of White Clover[J];Acta Agrestia Sinica;2005-01
7 Candidate: MA Chun-hui~1 Advisor: HAN Jian-guo~2(1.College of Animal Science and Technology, Tarim University, Alar, Xinjiang Uygur Autonomous Region 843300, China; 2.Institute of Grassland Science, China Agricultural University, Beijing 100094, China);Study on the Propagating Characters and Seed Production Technology of Zoysia japonica[J];Acta Agrestia Sinica;2005-02
8 XIA Xian jiu (Sichuan Animal Science Academy, Chengdu 610066, China);Introduction of herbage varieties to the Chengdu region[J];Pratacultural Science;2003-12
9 LIU Xin-bao~1, ZHANG Wan-jun~2, SHEN Yi-xin~1(1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Landscaping Company of Tianjin Economic and Technological Development Area, Tianjin 300457, China);Effect of paclobutrazol on white clover growth in the TEDA green belt[J];Pratacultural Science;2004-03
10 LIU Jian-xin,WANG Xin,WANG Feng-qin(Department of Life science,Longdong College,Qingyang 745000,China);Effect of water stress on osmotic adjustment and activity of protective enzymesin alfalfa seedlings[J];Pratacultural Science;2005-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved