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《Pratacultural Science》 2009-05
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Preliminary study on determination on alfalfa protein grouping

LI Rrui-qin,YU An-feng,SHUI Rong,XU Mei-rong,SONG Zheng-ping,ZHAO You-biao,JIAO Jie (Animal & Pasture Improvement Institute,Gansu Academy of Agriculture Science,Lanzhou 730070,China)  
Adopted the method of continuous extract on determination of grouping protein in alfalfa.The result showed that the alfalfa protein components varied with the type of extract reagents,their contents in order were 40%(v/v)Isopropanol2%(m/v)Sodium3.85%(v/v)Lactic acid0.5 %(m/v)Potassium.Proteins dissolved in alcohol,salt,acid and alkaline accounted for 14.50%,12.55%,7.73%,and 3.11% of the total crude protein,respectively.Others accounted for 63.11%.It was concluded that the most suitable extraction reagent for alfalfa leaf protein was dilute salt solution(0.5%).
【Fund】: 甘肃省自然科学基金暨中青年科技基金项目(YS991-A21-016);; 暨甘肃省农科院新产品研发专项(NKY-08-4)
【CateGory Index】: S541.9
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