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《Pratacultural Science》 2011-05
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Optimization of the ISSR reaction system of Elymus nutans by orthogonal design

LIU Xin-liang1,2,DE Ying1,ZHAO Lai-xi1(1.Grassland Research Institute,Chinese Academy of Agricultural Science,Inner Mongolia Hohhot 010010,China;2.Graduate School,Chinese Academy of Agricultural Science,Beijing 100081,China)  
Five factors(DNA polymerase,Mg2+,template DNA,dNTPs,primer) in ISSR-PCR reaction system were carried on the optimized experiment by taking the genomic DNA of Elymus nutans as template.The optimum reaction system and reaction process of E.nutans ISSR-PCR analysis were established.The results of this study showed that the volume of reaction system was 25 μL.It included 2.5 μL 10×buffer(except Mg2+),1.0 U TapDNA polymerase,2.0 mmol/L Mg2+,30-120 ng template DNA,0.25 mmol/L dNTP and 0.25 μmol/L primer.Eleven primers with stable amplification and rich polymorphism were obatined based on the optimal PCR reaction system.The reaction program was devised for 2 min at 94℃ in the first circle,1 min at 94℃,51℃ for 1 min(depended on different primer),72℃ for 1.5 min for 41 cycles,and 72℃ for 10 min for extending,which was obatined across the gradient PCR experiments,including temperature gradient,cycle gradient and extending time gradient experiments.Validation of the optimistic ISSR-PCR reaction system and amplification program showed that the reaction system and amplification program had high stability and good reproducibility.
【Fund】: 2008年度中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所 2008-Z-1);; 农业部“948”牧草种质资源收集与创新利用平台建设(2006-G8(4)-21);; 国家科技基础条件平台建设项目(2005DKA21007)
【CateGory Index】: S543.9
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2 LUO Yi-qi, LI Juan-ling, LIU Guo-min, CHENG Shan-han, PAN Xue-feng, ZAI Li-yan (Kudingcha Research Institute, Hainan University, Haikou 570228, China);RAPD Markers Linked to the Male Character of Ilex kudingcha C. J. Tseng[J];Journal of Tropical Organisms;2012-03
3 LI Meng-wei;LI Fang-fei;FAN Zhi-hong;YU Xiao-dan;LV Shu-xia;MA Di;College of Biological Science and Techology,Shenyang Agricultural University;;Optimization and Establishment of Inter-simple Sequence Repeat PCR Rreaction System for Lepista nuda[J];Biotechnology;2013-06
4 YU Jing 1,JING Zhao-bin 2,WEI Lin1,CHENG Ji-min3(1.College of Resources and Environment,Northwest A &F University,Yangling 712100,China; 2.College of Animal Science and Technology,Northwest A &F University,Yangling 712100, China;3.Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling 712100,China);Establishment and Optimization of the ISSR-PCR Reaction System in Stipa bungeana Trin.[J];Chinese Journal of Grassland;2012-01
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2 CHEN Zhi-hong et al (Center of Genetic Resources of Livestock,Foul and Herbage,China Animal Husbardry & Veterinary Institute,Beijing 100094);Establishment of Astragalus adsurgens' ISSR-PCR Optimal Conditions with Orthogonal Optimization Method[J];Journal of Anhui Agricultural Sciences;2006-13
3 LI Zhi-qiang(Animal Husbandry and Veterinary Bureau of Baiyin City,Baiyin 730900,China);Control of brown spot by mixed cultivation of Medicago sativa and Elymus nutans[J];Pratacultural Science;2009-10
4 LIU Rong,ZHANG Wei-guo,JIANG Xiao-lei,ZHANG Jun(College of Pastoral Agriculture Science and Technology,Lanzhou University,Gansu Lanzhou 730020,China);Study on the characteristics of degradation succession of Elymus nutans community and its correlation to soil properties[J];Pratacultural Science;2010-10
5 CHEN Zhi-hua1,2,MIAO Jia-min2,ZHONG Jin-cheng2,MA Xiao1,CHEN Shi-yong1,ZHANG Xin-quan1 (1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China);Genetic diversity of wild Elymus nutans germplasm detected by SRAP markers[J];Acta Prataculturae Sinica;2009-05
6 Xie Yunhai1* Xia De'an1 Jiang Jing1 Lin Ping2 1 Department School of Forest, Northeast Forestry University, Harbin, 150040; 2 Institute of Heilongjiang Forestry Research, Harbin, 150040;Optimization for ISSR-PCR System of Fraxinus mandshurica Rupr.Using Orthogonal Design[J];Molecular Plant Breeding;2005-03
7 Bai Jinjun Wei Anzhi Wang Jia Zhang Yazhuan Life Science College,Northwest A & F University,Yangling,712100;Optimization of an ISSR Analysis System of Apricot of Kernel-Consuming Based on Orthogonal Design[J];Molecular Plant Breeding;2009-06
8 GUI Teng-qin1,2,3,SUN Min2,3*,QIAO Ai-min4,WANG Xin-yan4 (1Deptment of Chemistry & Life Science, Qianxinan Teachers’College, Xingyi, Guizhou 562400 China; 2College of Life Science, South- west University, Chongqing 400715 China; 3Key Laboratory of Eco-environments (Ministry of Education) in Three Gorges Reservoir Region of Chongqing 400715 China; 4College of Agriculture and Landscape Architecture, Zhongkai University of Agriculture and Technology, Guangzhou, Guangdong 510225 China);Optimization of an ISSR reaction system of Japanese apricot (Prunus mume) based on orthogonal design[J];Journal of Fruit Science;2009-01
9 LI Rong-hua~1,XIA Yan-shi~2,LIU Shun-zhi~1,SUN Li-li~1, GUO Pei-guo~1, MIAO Shan-yu~1,CHEN Jin-hui~1(1.College of Life Sciences,Guangzhou University,Guangzhou 510006,China;2.College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China);CTAB-improved Method of DNA Extraction in Plant[J];Research and Exploration in Laboratory;2009-09
10 MA Xiao,ZHOU Yong-Hong,YU Hai-Qing,ZHANG Hai-Qin(Triticeae Research Institute,Sichuan Agricultural University,Dujiangyan 611830,China);Genetic Diversity of Gliadin in Wild Germplasm of Elymus nutans Griseb.[J];Hereditas;2006-06
Chinese Journal Full-text Database 10 Hits
1 MU Jin-hua,XU Wen-ling,ZHANG Yi-hui,WANG Cui-hua(Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Screening Resistance to Chinese Cabbage Soft Rot by Molecular Marker[J];Shandong Agricultural Sciences;2008-04
2 MA Rong-qun,SONG Zheng-xu,HUANG Yue,YUE Wen-hui*(Qingdao Institute of Agricultural Sciences,Qingdao 266100,China);Application Progress of Modern Biotechnology on Genetic Research of Welsh Onion(Allium fistulosum L.)[J];Shandong Agricultural Sciences;2009-06
3 Zhang Heshan Liu Yang(Institute of Animal Science,Hubei Academy of Agricultural Science,Wuhan,430209);Utilization and Development Foreground of Pueraria DC Resources[J];Anhui Agricultural Science Bulletin;2008-05
4 Zhao Yao et al.(Lu zhou City Agriculture Bureau,Luzhou 646000,China);The Application of SSR Markers in Seeds Quality Detection[J];Anhui Agricultural Science Bulletin;2011-07
5 CHEN Zhi-hong et al (Center of Genetic Resources of Livestock,Foul and Herbage,China Animal Husbardry & Veterinary Institute,Beijing 100094);Establishment of Astragalus adsurgens' ISSR-PCR Optimal Conditions with Orthogonal Optimization Method[J];Journal of Anhui Agricultural Sciences;2006-13
6 GUAN De-jun (Depatrment of Life Science,Anqing Normal University,Anqing,Anhui 246011);Principle of AFLP and its Application in Plant[J];Journal of Anhui Agricultural Sciences;2006-15
7 YANG Xi et al (Department of Grassland Science,Sichuan Agricultural University,Yaan, Sichuan 625014);Salt-Tolerance Appraisal and Comprehensive Evaluation of Wild Arrhenatherum elatius L.Seedling[J];Journal of Anhui Agricultural Sciences;2006-23
8 LIU Qing-shen et al(College of Animal Science,South China Agricultural University,Guangzhou,Guangdong 510642);Preliminary Study on the Genetic Background of Chinese Shar-pei in its Origin Place with RAPD Technique[J];Journal of Anhui Agricultural Sciences;2007-03
9 LIANG Yong-song et al (Agronomy College of Guizhou University,Guiyang,Guizhou 550025);Study on the High-efficiency and Comprehensive Cultivation Technology of Intercropping Gynostemma and Interplanting Mushroom in Maize Field[J];Journal of Anhui Agricultural Sciences;2007-25
10 FU Bing et al(Biotechnology Research Center of Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Genetic Diversity Analysis of 25 Tobacco Germplasm Resources by ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2008-03
China Proceedings of conference Full-text Database 10 Hits
1 HAN Guo-hui,WANG Wei-xing,XIANG Su-qiong,JIA zhi-gang,GUO Qi-gao,QIN Guo-xin, HE Qiao,LI Xiao-lin,LIANG Guo-lu~* (Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/College of Horticulture and Landscape Architecture.Southwest University,Chongqing 400715 China);Establishment and optimization of SCoT system in polyploidy loquats[A];[C];2011
2 XIE Guang-hui~(1,2*) ZHAO Ya-li~1 HAN Li-pu~1 DING Rong-e~3 CHENG Xu~(1,2) (1.Energy Crops Laboratory,College of Agriculture and Biotechnology,China Agricultural University,Beijing 100094,China;2.Center of Biomass Engineering,China Agricultural University,Beijing 100094,China;3.Division of Science and Technology,China Agricultural University,Beijing 100094,China);Perennial Herbaceous Lignin-Cellulosic Energy Crops and Potential Plant Resource the North China[A];[C];2007
3 LIU Rui~1 SUN Bao-wen~2 YANG Ji-shuang~2 (1 College of Landscape Architecture and Tourism,Hebei Agricultural University,Baoding 071001; 2 College of Horticulture,Hebei Agricultural University,Baoding 071001);Genetic Relationship of Edible and Ornamental Chrysanthemum Based on ISSR Analysis[A];[C];2009
4 Xie Lvwu,Guo Liang,Shi Hailiang,Jiang Xiaochuan,Zhao Hongyan (College of Urban and Environmental Science,Northeast Normal University,Changchun,130024);Orthogonal Test on Washing Aeolian Sandy Soil Contaminated by Crude Oil[A];[C];2010
5 ;DNA Extraction from Polygonum multiflorum thunb and Orthogonal Design Method for Optimal RAPD Reaction System[A];[C];2011
6 ;Morphological Diversity of the Ear traits of Elymus nutans Griseb L. in the North-western Part of Sichuan plateau[A];[C];2007
7 Lu Jing-wei Wang Zan Zhang Xin-qiuan Yang Xi Gao Hong-wen Zhang Xin-yu Zhai Chun-mei;Study on Phenotypic Diversity of Caragana Microphylla Lam. Natural Polpulations in the middle of Inner Mongolia[A];[C];2007
8 ZHENG Yi-qi~(1,2) WANG Zhi-yong~2 GUO Hai-lin~2 XUE Dan-dan~2 LIU Jian-xiu~(2*) (1.Department of Horticulture Nanjing Agricultural University Nanjing 210095 China; 2.Institute of Botany Jiangsu Province and Chinese Academy of Sciences Nanjing 210014 China);Optimization of SRAP-PCR System on centipedegrass Using Orthogonal Design and Selection of Primers[A];[C];2007
9 Huang Lin-kai Zhang Xin-quan Li Fang Ma Ke (Department of Grassland Science College of Animal Science and Technology Sichuan Agricultural University Sichuan Ya'an 625014 China);Clonal diversitv and CIonal structure of Hemarthria compressa[A];[C];2007
10 Zhong Li~(1,2),Wang Xiao-Li~2,Gan You-Min~1,Tang Cheng-Bin~2,Shang Yi-Shun~2; (1.Department of Grassland Sichuan Agricultural University Ya'an 625014; 2.Guizhou Institute of prataculture Dushan 558200);ISSR Analysis on Genetic Diversity of Wild Agrostis stolonifera L.Germplasm Resources[A];[C];2007
Chinese Journal Full-text Database 10 Hits
1 ZHAO Wen-xiu et al(Staff Room of Chemistry,Jilin Medical College,Jilin 132013);Determination on 10 Metal Element Contents in Kudingcha and Preliminary Exploration on Its Health Function[J];Journal of Anhui Agricultural Sciences;2008-06
2 JIN Lin et al(School of Life Sciences and Food Engineering,Nanchang University,Nanchang,Jiangxi 330031);Optimization of ISSR-PCR Reaction System for Miscanthus floridulus[J];Journal of Anhui Agricultural Sciences;2009-29
3 HAO Ming-ming et al(Biotechnology Research Center/Hubei Province Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang,Hubei 443002);Analysis of Chromosome Karotype of Miscanthus sinensis[J];Journal of Anhui Agricultural Sciences;2010-01
4 LIU Xin et al(Key Laboratory of Marine Genetics and Breeding of Chinese Ministry of Education,Ocean University of China,Qingdao,Shandong 266003);New Advances in the Development of Molecular Markers[J];Journal of Anhui Agricultural Sciences;2011-23
5 Amit SHARMA, Gaurav ZINTA, Satender RANA, Poonam SHIRKOT Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture & Forestry, Nauni, Solan 173230 (Himachal Pradesh), India;Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers[J];中国林学(英文版);2010-02
6 YANG Shui-lian,LIU Wei-dong,MA Tao,SHU Bi-chao,LIU Rui-feng,XIE Peng(Central South University of Forestry and Technology,Changsha 410004,China);Establishment and optimization of ISSR-PCR reaction system of centipedegrass[J];Grassland and Turf;2009-01
7 LIU Li-xiang1, LIANG Xing-fei1, SUN Yi1, Tanguy Laura1,2, YE Hong1, ZENG Xiao-xiong1* (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Department of Food Engineering, Polytech’Lille, 59655 Villenevve d’Asqc, France);Determination of Polyphenolic Content and Antioxidant Activity of Kudingcha Made from Ilex kudincha C. J. Tseng[J];Journal of Tea Science;2008-04
8 GUO Lian-yun,ZHAO Nian-wu,TIAN Hui-chun(Meteorological Bureau in Hainan State of Qinghai Province,Qinghai Gonghe 813000,China);Impacts of climatic warming on reproductive stages of forages growing in alpine grassland of the Three River Sources Areas[J];Pratacultural Science;2011-04
9 SUI Xiao-qing,WANG Kun (Institute of Grassland Science,College of Animal Science and Technology,China Agriculture University,Beijing 100094,China);Establishment and optimization of the ISSR-PCR reaction system in Stipa krylovii[J];Acta Prataculturae Sinica;2008-03
10 GONG Yan-ming1,2,HU Yu-kun1,ADELI Mai-dil1,LI Kai-hui1,YIN Wei1,2,ZHANG Wei1,2,WANG Ji-yun3(1.Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Changji Station of Prairie,Changji 831100,China);Analysis of adaptation of a climate productivity model on alpine grassland[J];Acta Prataculturae Sinica;2010-02
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 YU Jing1,JING Zhao-bin2,WEI Lin1,CHENG Ji-min3(1.College of Resources and Environment,Northwest A &F University,Yangling 712100,China; 2.College of Animal Science and Technology,Northwest A &F University,Yangling 712100,China; 3.Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling 712100,China);ISSR analysis of genetic differentiation of Stipa bungeana populations in the mountain area of southern Ningxia[J];Pratacultural Science;2012-12
2 QIAN Xin;LIAN Jing-Jing;LIU Fen;NIU Xiao-Ling;WANG Cai-Xia;TIAN Min;Research Institute of Subtropical Forestry,Chinese Academy of Forestry;South China Botanical Garden,Chinese Academy of science;Administration Bureau of Tianmu Mount National Nature Reserve;;Establishment and Optimization of ISSR-PCR Reaction System for Calanthe tsoongiana[J];Bulletin of Botanical Research;2013-06
3 LI Yu-ying;FAN Xi-feng;HOU Xin-cun;ZHU Yi;ZHAO Chun-qiao;WU Ju-ying;Beijing Research and Development Center for Grass and Environment/Key Laboratory of Urban Agriculture (North)of Ministry of Agriculture,Beijing Academy of Agriculture and Forestry Sciences;College of Agronomy and Biotechnology,China Agricultural University;;Genetic diversity of Miscanthus &Triarrhena based on ISSR and SSR analysis[J];Journal of China Agricultural University;2014-02
4 DAI Wen-juan1,LI Qian-kun1,2,LUO Wen-hua1,TANG Wen-xiu1,ZHAO Bo1,PAN Bo1,HUANG Shi-xun1(1.Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and the Chineses Academy of Sciences,Guilin Guangxi 541006,China;2.Guilin University of Technology,Guilin Guangxi 541004,China);The Optimization of RAPD-PCR System of Hopea chinensis[J];Seed;2013-07
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jian-bo et al (Guizhou Institute of Prataculture,Dushan,Guizhou 558200);Study on Gliadin Genetic Trait of Wild Elymus nutans in the Northwestern Plateau of Sichuan Province[J];Journal of Anhui Agricultural Sciences;2008-10
2 Zhang Deqiang;Zhang Zhiyi;Yang Kai.Institute of Populus tomentosa, Beijing For.Univ., 100083, P.R.China.\;;Applications of AFLP technique to genetic improvement in forest trees.[J];Journal of Beijing Forestry University;2000-06
3 Zhang Chunxiao Li Yue Shen Xihuan (College of Forest Resources and Environment,Beijing For.Univ.,100083,P.R.China);A Review on Forest Trees Genetic Diversity at the Isozyme Level[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-03
4 Yu Zhuo; Yun Jinfeng(Inner Mongolia Agricultural University, Hohhot 010019)Ma Youzhi Xin Zhiyong(Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081);RAPD Analysis on the Genomic DNA of Several Triticeae Grasses and Their Hybrids[J];ACTA AGRESTIA SINICA;1999-04
5 Li Zaozhe Yun Jinfeng Ma Qingzhi Yu Zhuo (Inner Mongolia Agricultural University,Huhhot 010019);Analysis of Esterase Isozymes of Elymus dahuricus,Hordeum brevisubulatum Their Hybrid F 1 and BC 1[J];ACTA AGRESTIA SINICA;2000-03
6 YUAN Qing hua, ZHANG Wen shu, LI Min (Institute of Animal Science, CAAS, Beijing 100094,China);Study on Excised Leaf Tissue Inoculation of Common Leaf Spot in Alfalfa[J];Acta Agrestia Sinica;2001-01
7 YU Zhuo,SONG Yong fu,LI Zao zhe,YUN Jin feng (Inner Mongolia Agricultural University,Huhhot010019,China);Study on Growth,Development and Cytogenetics of Elymus canadensis×E.dahuricus Hybrid F_1[J];Acta Agrestia Sinica;2002-04
8 DONG Ai-xiang, HU Lin, ZHAO Mei-qi, ZHANG Fu-suo, CHEN Shi-qi (China Agricultural University,Beijing, 100094 China);Resistance Against Brown Patch of Different Poa pratensis Cultivar[J];Acta Agrestia Sinica;2003-01
9 LIU Jianxiu, GUO Aigui, GUO Hailin(Institute of Botany, Chinese Academy of Sciences of Jiangsu, Nanking,Jiangsu Province 210014, China);The Preliminary Study on the Morphological Variations and Morphological Types of Zoysia sinica in China[J];Acta Agrestia Sinica;2003-03
10 LIU Jian-xiu, ZHU Xue-hua, GUO Ai-gui, GUO Hai-lin (Institute of Botany, Chinese Academy of Sciences of Jiangsu Province,Nanjing,Jiangsu Province 210014, China);Morphological Variations of Eremochloa Ophiuroides and its Morphological Types[J];Acta Agrestia Sinica;2004-03
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