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《Pratacultural Science》 2011-05
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Cloning and sequencing of GAPDH gene from Vicia sativa

ZHANG Lei,LIU Zhi-peng,ZHANG Ji-yu,ZHANG Miao-qing,WANG Yan-rong(College of Pastoral Agricultural Science and Technology,Lanzhou University,Gansu Lanzhou 730020,China)  
To provide molecular basis of high-altitude adaption,a partial sequence of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was isolated from common vetch(Vicia sativa).In this study,a pair of primers was designed according to homologous GAPDH genes among closely related plants,and then the reverse transcription PCR(RT-PCR) was performed.A nucleotide sequence of 1 141 bp with coding 381 animo acides was identified.Homological analysis showed that the similarity between this sequence and GAPDH gene sequences of other organisms was over 83% in nucleoride sequence level and over 95% in animo acid level.Therefore,the sequence was considered to be V.sativa GAPDH gene.It was named VsGAPDH,and was submited to GenBank with accession number HM117006.
【Fund】: 国家基础研究发展计划973项目(2007CB108904);; 兰州大学中央高校基本科研业务费专项资金(lzujbky-2009-4);; “十一五”国家科技支撑计划子课题(2008BADB3B07)
【CateGory Index】: S551.9
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