Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pratacultural Science》 2011-05
Add to Favorite Get Latest Update

Cloning and sequencing of GAPDH gene from Vicia sativa

ZHANG Lei,LIU Zhi-peng,ZHANG Ji-yu,ZHANG Miao-qing,WANG Yan-rong(College of Pastoral Agricultural Science and Technology,Lanzhou University,Gansu Lanzhou 730020,China)  
To provide molecular basis of high-altitude adaption,a partial sequence of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was isolated from common vetch(Vicia sativa).In this study,a pair of primers was designed according to homologous GAPDH genes among closely related plants,and then the reverse transcription PCR(RT-PCR) was performed.A nucleotide sequence of 1 141 bp with coding 381 animo acides was identified.Homological analysis showed that the similarity between this sequence and GAPDH gene sequences of other organisms was over 83% in nucleoride sequence level and over 95% in animo acid level.Therefore,the sequence was considered to be V.sativa GAPDH gene.It was named VsGAPDH,and was submited to GenBank with accession number HM117006.
【Fund】: 国家基础研究发展计划973项目(2007CB108904);; 兰州大学中央高校基本科研业务费专项资金(lzujbky-2009-4);; “十一五”国家科技支撑计划子课题(2008BADB3B07)
【CateGory Index】: S551.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Lian,ZHANG Li-ping,WANG Lei,WANG Jia,WU Jian-ping (Faculty of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China);Molecular cloning and bioinformatics analysis of MT-IV in the yak[J];Pratacultural Science;2008-10
2 ZHANG Jianquan,NAN Zhibiao, WANG Yanrong(College of Pastoral Agriculture Science and Technology, Lanzhou University;Gansu Grassland Ecological Research Institute, Lanzhou 730020, China);POD isozyme analysis for F_0 and F_3 of four Vicia sativa lines[J];Acta Pratacultural Science;2003-05
3 WANG Yan-rong, NAN Zhi-biao, NIE Bin, ZHANG Jian-quan, ZHANG Ji-yu(College of Pastoral Agriculture Science and Technology of Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020, China);Distinct comparison of morphological characteristics for several new cold resistant Vicia sativa lines[J];Acta Pratacultural Science;2005-02
4 WANG You-ning~(1,2),LIU Meng-yu~2,LI Xia~(2*) (1 Research Center of Agricultural Sources,Insititute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China;2 Graduate School Chinese Academy of Sciences,Beijing 10039,China);Multidimensional Nature of Glyceraldehyde-3-phosphate Dehydrogenase in Plants[J];Acta Botanica Boreali-occidentalia Sinica;2005-03
5 CAI Hua,ZHU Yan-ming,BAI Xi,LI Yong,JI Wei(College of Life Science,Northeast Agricultural University,Harbin 150030,China);Cloning and analysis of a novel DREB gene of Glycine soja[J];Pratacultural Science;2009-08
6 ZHOU Ling-ling1,MIAO Jian-kun1,ZHU Jian-bo1,CAO Lian-pu2 (1.Key Laboratory of Agriculture Biological Technology of College of Life-Science,Shihezi University,Shihezi 832000,China;2.College of Agriculture,Shihezi University,Shihezi 832000,China);Cloning and sequence analysis of a Na~+/H~+ antiporter gene in the halophyte Limonium gmelinii[J];Acta Prataculturae Sinica;2009-05
7 CUI Run-li2,4,WANG Yong-fang1,ZHI Hui1,3,LI Wei1,LI Hai-quan1,HUANG Zhan-jing4,DIAO Xian-min1,3(1.Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;2.Department of Pharmaceutical Engineering,Hebei Chemical Medicine Vocational and Technical College,Shijiazhuang 050026,China;3.Institute of Crop Sciences,Chinese Academy of Agriculture Sciences,Beijing 100081,China;4.College of Life Sciences,Hebei Normal University,Shijiazhuang 050012,China);Cloning and Structure Analysis of Foxtail Millet APDH Gene[J];Acta Agriculturae Boreali-Sinica;2009-03
8 LIU Zhi-hua YANG Qian* (Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001,China);Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase gene from Chaetomium globosum[J];Acta Microbiologica Sinica;2005-06
9 Yue Caifeng, Kang Guozhang, Liu Chao, Guo Tiancai, Zhu Yunji, Shen Bingquan (National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002);Cloning and Sequencing of GAPDH Gene in Common Wheat(Tritucum aestivum L.)[J];Chinese Agricultural Science Bulletin;2008-04
10 LI Xiao-Ze, LIU Guan-Jun, YANG Chuan-Ping* The Provincial Key Laboratory of Forest Genetics and Breeding, The Laboratory of Forest Genetics and Breeding and Biotechnol-ogy of Ministry of Education, Northeast Forestry University, Harbin 150040, China;Cloning and Sequence Analysis of cDNA of Glyceraldehyde-3-phosphate Dehydrogenase Gene from Polygonum sibiricum Laxm.[J];Plant Physiology Communications;2007-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yan-rong, NAN Zhi-biao, NIE Bin, ZHANG Jian-quan, ZHANG Ji-yu(College of Pastoral Agriculture Science and Technology of Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020, China);Distinct comparison of morphological characteristics for several new cold resistant Vicia sativa lines[J];Acta Pratacultural Science;2005-02
2 YUAN Qing-hua~1,ZHANG Ji-yu~2,ZHANG Wen-shu~1(1.Institute of Animal Science,CAAS,Beijing 100094,China;2.College of Pastoral Agriculture Science and Technology of Lanzhou University,Gansu Grassland Ecological Research Institute,Lanzhou 730020,China);Allozyme analysis of genetic diversity of wild Lespedeza[J];Acta Prataculturae Sinica;2006-05
3 ZHANG Ya-guan1,XUE Shi-ming2,KUANG Chong-yi2,CHEN Gong1(1.Department of Grassland Science,Yunnan Agriculture University,Kunming 650201,China;2.Yunnan Beef Cattle and Pasture Research Center,Kunming 650212,China);Relation grade analysis of grey theory and comprehensive evaluation of introduced forage of winter-free farmland in the north of Yunnan subtropics[J];Acta Prataculturae Sinica;2007-03
4 XING Yi,ZHAO Xiang,DONG Kuan-hu,SHI Wei-wei (College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China);A study on morphological variation of different populations of Lespedeza davurica[J];Acta Prataculturae Sinica;2008-04
5 LU Bing-guo1,HE Wei-yi1,SHEN Yan-hong1,JIANG Ji-mou2, CHEN Xiao-jing1,CHEN Wei3,L Liu-xin1(1.College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;3.College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Cloning and sequence analysis of the full-length cDNA of glyceral-dehyde-3-phosphate dehydrogenase gene from longan cotyledon embryo[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2009-05
6 Wei Mingming Wang Junsheng Zhang Gaisheng Zhang Longyu Yuan Zhengjie Sun Rui Ye Jingxiu Niu Na Ma Shoucai Li Hongxia Key Laboratory of Crop Heterosis and Utilization Research of Shaanxi Province, Northwest A & F University, Wheat Breeding Engineering Research Center, Ministry of Education, Yangling, 712100;Relationship Between the Expression of GAPDH Gene and Anther Abortion of Physiological Male Sterile of Wheat[J];Molecular Plant Breeding;2009-04
7 Wang Xiaoli 1,2 Liu Xiaoxia 1 Li Wanchen 2 Wu Jiahai 1 Yang Yicheng1 Chen Wei 1 Wang Shuying 1 1 Guizhou Institute of Prataculture,Guiyang,558200; 2 Maize Research Institute,Sichuan Agricultural University,Ya'an,625001;Cloning and Characterization of Glyceraldehyde-3-Phosphate Dehydrogenase Gene FaGAPDH in Tall Fescue[J];Genomics and Applied Biology;2010-02
8 XU Chun-bo1,WANG Yong2,LI Xing-you1,ZHAO Hai-xia1(1.Grassland Research Institute,Chinese Academy of Agricultural Sciences,Inner Mongolia Huhhot 010010,China;2.College of Ecology and Environment,Inner Mongolia Agricultural University,Inner Mongolia Huhhot 010018,China);Construction of plant expression vector containing Arabidopsis transcription factor CBF4[J];Pratacultural Science;2010-06
9 Yu Lili,Gao Caiqiu,Wang Yucheng,Yao Qichao,Liu Guifeng,Yang Chuanping(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University),Ministry of Education,Harbin 150040,P. R. China); Liu Yan(Ji'an Agricultural Bureau,Jilin Province);Cloning and Expression of a Glyceraldehyde-3-phosphate Dehydrogenase Gene from Tamarix hispida[J];Journal of Northeast Forestry University;2010-07
10 LI Hong-yan,ZHENG Qing-song,JIANG Chao-qiang,LIU Zhao-pu,LIU Guo-hong,XIN Ben-rong (College of Resources and Environmental Sciences,Key Laboratory of Marine Biology Jiangsu Province,Nanjing Agricultural University,Nanjing 210095,China);A comparison of stress effects between chloridion and sodium ion on grain amaranth seedlings under NaCl stress[J];Acta Prataculturae Sinica;2010-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Zhong 1, TANG Xue feng 2, WANG Zhi hong 2 (1.Gansu Animal Husbandry Technology Development Center, Lanzhou 730030, China; 2.Sunan County Grassland Management Station, Gansu Sunan, 734400, China);Influence of multiple cuttings on productivity of Vicia sativa sown in cold alpine pastoral regions[J];PRATACULTURAL SCIENCE;1999-05
2 ZHANG He-ping;HU Jiang-lin;WANG Xing-long;WEI Jian(Zhangye Grassland Station,Zhangye Gansu 734000,China);Effect of 'Yiwei' yield-increased bacteria on Vicia sativa sowed in autumn[J];PRATACULTURAL SCIENCE;1999-06
3 Ma Chun hui 1, Han Jian guo 2 (1. Animal Science Campus, Tarimu Agricultural and Reclamation University, Alar 843300, Xinjiang, China; 2. Grassland Research Institute, China Agricultural University, Beijing 100094, China);A study on the optimal harvest time of annual forage crops[J];Pratacultural Science;2001-03
4 MUNG Xian-zheng1,MUNG Hai-bo1,WANGJian-qiang2,ZHAO Rong-xia2,YANG Pen-yu2(1.Foreign Aid Dairy Project Office,Gansu Province,Lanzhou 730030,China;2.Gansu Provincial Grassland Monitoring Station,Lanzhou 730046,China);Systematic thoughts on Qinghai-tibetan Plateau to establish grassland-dairy yak ecological system[J];Pratacultural Science;2005-11
5 SHENG Hui,ZHU Yan-ming,LI Jie,BAI Xi,CAI Hua (Northeast Agriculture University,Harbin 150030,China);Genetic transformation of DREB2A gene into alfalfa[J];Pratacultural Science;2007-03
6 ZHAI Gui-yu1,2,SHEN Yi-xin2,LIU Xin-bao1,JIANG Hui-xin1,2,GAO Chen3 (1.Shandong Animal Husbandry and Veterinary Station,Jinan 250022,China;2.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;3.College of Information Engineering,Beijing Normal University,Beijing 100092,China);Characteristics of growth,development and dry matter accumulation of wild soybean under cultivated condition[J];Pratacultural Science;2008-10
7 Shuai Surong Zhang Xinquan (Sichuan Agricultural University, Yaan 625014) Bai Shiqie (Sichuan General Grassland Station, Chengdu 610041);ESTERASE AND PEROXIDASE IN DIPLOID AND TETRAPLOID COMMON ORCHARDGRASSES[J];PRATACULTURAL SCIENCE;1998-06
8 WANG Yun wen 1, NAN Zhi biao, WANG Yan rong 1, LI Chun jie 1, MA Long xi 2 (1. Gansu Grassland Ecological Research Institute, Lanzhou, Gansu, 730020, China; 2. Grassland Management Station of Xiahe County, Xiahe, Gansu, 747100, China);Herbage and seed yields of Vica and Lathyrus species under alpine grassland conditions[J];Acta Pratacultural Science;2001-02
9 REN Jian1,MAO Kai2, WU Yan-qi2 (1.Department of Grassland Science, Yunnan Agricultural University, Kunming 650201, China; 2.Department of Grassland Science, Sichuan Agricultural University, Yaan 625014, China);Comparison of peroxidase isozymes and esterase isozymes in Sichuan's native centipedegrass[J];Acta Pratacultural Science;2001-03
10 LI Zao-zhe, MA Qing-zhi, YUN Jin-feng, YU Zhuo (College of Eco-Environment, Inner Mongolia Agricultural University, Huhhot 010019, China);Analysis of peroxidase isozymes of Elymus dahuricus, Hordeum brevisubulatum and their hybrid F_1 and BC_1[J];Acta Pratacultural Science;2001-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved