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《Pratacultural Science》 2011-06
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Isolation and detection of swainsonine from Oxytropis glabra in the southern Xinjiang region

WANG Shuai,WU Shu-qi,HU Jian-jun,ZHANG Ling,MA Chun-hui (Key laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production & Construction Corps;College of Animal Science,Tarim University,Xinjiang Alar 843300,China)  
The thin hydrochloric acid was used to extract the filtration from Oxytropis glabra,and this filtration was applied to get the crude alkaloids by eluting with 1 mol/L ammonia water on 732 strong eation-exchange resins.White crystal was obtained from the crude alkaloids by the following steps,extracted by methanol,then applied to silica gel stele(180 μm),eluted with chloroform∶methanol∶ammonia∶water(70∶26∶2∶2).The crude alkaloids was monitored by thin-layer chromatography,incorporated and evaporated the same fraction,finally,the white crystal was obtained under vacuum(-0.094 MPa) at 90 ℃.The white crystal was possibly considered as swainsonine,the chemical structures of which were identified to further confirm that this white crystal was swainsonine by means of TLC,MP,UV,IR,MS and NMR.The extraction of swainsonine in O.glabra was 19.95 mg/kg.
【Fund】: 新疆生产建设兵团博士基金项目(08BB08);; 国家自然科学基金项目(31060024)
【CateGory Index】: S452
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