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《Pratacultural Science》 2012-07
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Effects of explants and nitrogen concentration on callus induction of Glycyrrhiza uralensis

LIU Fu-zhi1,LIN Hai-ming1,LI Zhan-qiang1,ZHANG Han-ping2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China; 2.Graduate School on Liquorice Biology-breeding of Jiuquan,Jiuquan 735000,China)  
An experiment was conducted to determine the effects of different explants and nitrogen concentration on callus induction of Glycyrrhiza uralensis.The tissue culture method was used to compare the effects of root,stem,cotyledon and hypocotyls on callus induction and growth.The results of this study showed that the callus of hypocotyls was induced by higher induction rate,quicker induction time and lower browsing rate,indicating that the hypocotyls was optimal explants to induce the callus.The different concentration of nitrogen had the most significant effects on callus growth and induction of hypocotyls in certain hormone concentration,in which the lower and higher nitrogen concentration had negative effects on the growth of callus,and the optimal nitrogen concentration was 60 mmol·L-1,which had the best effects on callus growth and induction.The different explants and nitrogen concentration were the important factors affecting the growth and induction of callus.
【Fund】: 甘肃省教育厅基金项目(0902-03);; 甘肃省农牧厅科技攻关项目(gyc09-06);; 国家科技支撑计划(2011BAI05B01)
【CateGory Index】: S567.71
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