Effects of tonoplast Na~+/ H~+ antiporters gene silence on leaf stomata characteristics of Zygophyllum xanthoxylum
YUAN Hui-jun;MA Qing;WEI Li;HU Jing;WANG Pei;WANG Suo-min;State Key Laboratory of Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University;College of Life Science and Engineering,Lanzhou University of Technology;
The stomata characteristics of different Zygophyllum xanthoxylum lines including wild type( WT) and ZxNHX-silenced lines( L2 and L7) using post-transcriptional gene silencing were analyzed. Under 50 mmol·L-1Na Cl treatment,4 more hours was required for stomatal aperture of Zx NHX-silenced lines reached to peak value compared with WT. Under-0. 5 MPa osmotic stress treatment,the peak value of stomatal aperture in L7 lines significantly decreased by 12% compared with that of WT. Under control conditions,the stomatal size of L7 lines significantly reduced and 6 more hours was required for stomatal aperture reached to peak value compared with WT. Leaf tissue water contents in Zx NHX-silenced lines were always significantly lower than that in WT under all the treatments,especially under 50 mmol·L-1Na Cl treatment,leaf tissue water content of L7 lines was 40% less than that of WT. These results suggested Zx NHX regulated stomatal activities by controlling leaf water status of Z. xanthoxylum.