Effect of PEG on germination and active oxygen metabolism in wildrye ( Leymus chinensis )seeds
LIU Jie, LIU Gong she, QI Dong mei, LI Fang fang, WANG En hua (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)
The effects of osmotic priming on germination and vigour of wildrye seeds treated with polyethylene glycol 6000 (PEG 6000) in different priming osmotic potentials and time durations were discussed in this paper. The results revealed that the germination rate and vigour index of wildrye seed pretreated with PEG increased obviously, whereas the conductivity rate decreased comparing to the control. During germination period, although the respiratory intensity of wildrye seeds pretreated with 30% PEG for 24 h was lower at beginning, it increased rapidly soon afterward; the content of O 2 decreased visibly but the activity both of superoxide dismutase (SOD) and peroxidase (POD) increased. The accumulation of malondialdehyde (MDA) was much lower than that of control. Consequently, it suggested that there was some interacting relationship between the increasing of vigour index through pretreatment with PEG and the increasing of activity oxygen metabolism.