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Changes of carbohydrate contents in below ground organs of Leymus chinensis and Stipa grandis in the inner Mongolian steppes

ZHANG Guang-hui~(1.2),LI Zeng-jia~2,PAN Qing-min~1,Ning Tang-yuan~2,YANG Jing-cheng~3(1.Lab of Quantitative Vegetation Ecology,Institute of Botany,the Chinese Academy of Sciences, Beijing 100093,China;2.Agronomy College of Shandong Agriculture University,Tai'an 271018,China;3.Beijing Museum of Natural History,Beijing 100093,China)  
Perennial grasses store enough carbohydrates in below ground organs for germination in spring or for regrowth after cutting.Leymus chinensis and Stipa grandis are dominant species with a wide distribution in the Inner Mongolian steppes.To understand the characteristics of carbohydrate storage of perennial grasses,we analyzed the content and seasonal dynamics of carbohydrate components in below ground organs of L.chinensis and S.grandis using high performance liquid chromatography.The results showed that the carbohydrate components in below ground organs of both L.chinensis and S.grandis included fructan,sucrose,glucose,fructose,mannitol and starch.The major components of below ground organs of both grasses were fructan,mannitol and starch and L.chinensis had much higher levels than S.grandis.The fructan and starch contents of L.chinensis were 3.2 and 3.6 times respectively those of S.grandis.The seasonal changes of total carbohydrate,soluble carbohydrate,starch,mannitol and fructan in the below ground organs of both species showed the same 'down-up-down-up' trend.Different carbohydrate components had different starting and ending times for accumulation before winter and for cleavage after spring.
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