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《Acta Prataculturae Sinica》 2006-05
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Genetic diversity of wild Dactylis glomerata in China detected by inter-simplesequence repeat(ISSR) molecular markers

FAN Yan~1,ZENG Bing~2,ZHANG Xin-quan~2,MA Xiao~2(1.Chongqing Animal Science Research Institute,Chongqing 400039,China;2.Department of Grassland Sichuan Agricultural University,Ya'an 625014,China)  
In a comparison of Dactylis glomerata cv.Baoxing with D.glomerata cv.Amba,Inter-simple Sequence Repeat(ISSR) molecular markers were used to detect the genetic diversity among 30 specimens of D.glomerata.Collected from Sichuan,Chongqing,Yunnan,Guizhou,and Xinjiang provinces.The twelve primers selected for use and produced a total of eighty-eight polymorphic bands,an average of 7.3 bands from each pair of ISSR primers.The average percentage of polymorphic bands was 83.8%,and the range of GS was(0.692 0)-0.926 0,which indicated a rich genetic diversity of D.glomerata in China.Based on cluster and principal component analyses of the genetic characteristics,the wild D.glomerata could be divided into 5 groups according to the nearest phylogenetic relationship.In most cases materials from the same region were in the same group thus showing a geographical distribution of genetic diversity of the tested materials.Advice is given on the collection and conservation of germplasm resources in wild D.glomerata populations.
【Fund】: 国家自然科学基金项目(30371022);; 重庆市科委基金项目(2004BB1240)资助
【CateGory Index】: S543.3
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