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《Acta Prataculturae Sinica》 2006-05
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Allozyme analysis of genetic diversity of wild Lespedeza

YUAN Qing-hua~1,ZHANG Ji-yu~2,ZHANG Wen-shu~1(1.Institute of Animal Science,CAAS,Beijing 100094,China;2.College of Pastoral Agriculture Science and Technology of Lanzhou University,Gansu Grassland Ecological Research Institute,Lanzhou 730020,China)  
Genetic diversity was estimated by vertical slab polyacrylamide gel electrophoresis in fourteen wild Lespedeza populations from Beijing and surrounding regions.With improved complexity Tris-HCl extraction buffer,seven enzymes,including 18 loci were assessed for allozyme genetic diversity.The percentage of polymorphic loci(P),average allele number(A),mean observed heterozygosity per locus(Ho),expected heterozygosity per locus(He),Shannon's information Index(I),ratios of gene diversities of heterozygosities(F_(ST)) and gene flow(N_m) of 14 Lespedeza populations were 54.62%,1.743 7,0.384 5,0.259 8,0.378 2,(0.447 6) and 0.309 6,respectively.Results of allozyme cluster analysis based on Nei's genetic distance agreed with the results of morphological cluster analysis.
【Key Words】： Lespedeza allozyme wild population genetic diversity
【Fund】： 国家科技部基础平台项目(2005DKA21007-6-2);; 农业部保种基金项目(2005-1)资助
【CateGory Index】： S541.5
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