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《Acta Prataculturae Sinica》 2007-01
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Genetic diversity in Festuca arundinaces based on random amplified polymorphic (RAPD) DNA analysis

TIAN Zhi-hong,QIU Yong-fu,YAN Han,HE Yong(College of Life Science,Yangtze University,Jingzhou 434025,China)  
Fifteen varieties of F.arundinaces introduced from foreign countries were assessed for genetic diversity by RAPD DNA analysis using twelve primers selected from 60 arbitrary primers.They amplified a total of 85 DNA bands of which 59 DNA bands(69.41%) were polymorphic: the average number of polymorphic DNA bands amplified by each primer was 4.92.The range of Jaccard's genetic similarity coefficient among these varieties varied from 0.373 to 0.932 sa assessed by NTSYS-PC software.A DNA molecular dendrogram was established for 15 varieties of F.arundinaces based on UPGMA analysis of the genetic similarity matrix.Three groups were idnetified based on a standard of 0.68 similarity coefficient in the dendrogram,"Triplet" and "Pixie" each constituted a group and the other varieties clustered together as a third group.
【Fund】: 湖北省重点科技发展计划项目(992P0603);; 湖北省教育厅重大科研项目(99Z007);; 湖北省新世纪高层人才工程科研项目(鄂人2003-31)资助
【CateGory Index】: S688.4
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