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《Acta Prataculturae Sinica》 2007-03
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Genetic diversity of bermudagrass accessions in south-west China by ISSRs molecular markers and geographic provenance

LIU Wei1,ZHANG Xin-quan1,LI Fang1,MA Xiao1,FAN Yan2(1.Department of Grassland,Sichuan Agricultural University,Ya'an 625014,China;2.Institute of Animal Science and Veterinary Medicine,Chongqing 400039,China)  
The genetic diversity of 42 native bermudagrass accessions and 3 cultivars in south-west China was investigated using 66 ISSR primers of which only 11 generated distinct amplified products.Among the 45 accessions of bermudagrass,a total of 82 bands were detected by the 11primers,and each primer amplified 5 to 10 bands(mean of 7.45 bands).Fifty eight of the 82 bands(70.9%) were polymorphic(mean of 5.27 polymorphic bands per primer).The ISSR-based genetic similarity(GS) values among 45 bermudagrass accessions ranged from 0.69 to 0.98,and among the 42 native bermudagrass accessions was from 0.77 to 0.98,indicating a rich genetic diversity of native bermudagrass accessions and certain differences from cultivars in south-west China.Cluster analysis showed that all the 45 accessions could be distinguished by ISSR markers and divided into 4 groups according to the nearest phylogenetic relationship.Among the 45 accessions,3 cultivars were classified in one group,and the other native bermudagrass accessions were classified in another 3 groups.Correlation analysis of between the genetic distance matrix and the original geographic distance matrix showed that the genetic relationships among native bermudagrass accessions based on ISSR markers was not strictly correlated with the geographical provenance.
【Fund】: 四川省重点青年基金项目(02ZQ026-057);; 四川省“十一五”饲草育种攻关项目(06SG023-001)资助
【CateGory Index】: S688.4
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