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《Acta Prataculturae Sinica》 2007-04
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Genetic diversity of Hemarthria compressa germplasm detected by inter-simple sequence repeat(ISSR)

FAN Yan1,2,LI Fang1,ZHANG Xin-quan1,MA Xiao1(1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.Chongqing Municipal Institute of Animal Husbandry,Chongqing 400039,China)  
The inter-simple sequence repeat(ISSR) method was applied to detect genetic variation of 28 Hemarthria compressa accessions from Southwest China(Sichuan province,Chongqing city and Guizhou province).Thirteen primers were selected from 96 ISSR primers,and 129 DNA fragments were amplified from 28 samples.Of these,110 fragments(84.2%) were polymorphic and the average number of DNA bands was 9.9 per primer.The genetic similarity among all accessions ranged from 0.466 to 0.980,which indicated that the genetic diversity was comparatively rich among the accessions tested.UPGMA clusters based on genetic similarity and principle component analysis(PCoA) based on band patterns divided the accessions into two groups corresponding to their geographical sources.The application of ISSR analysis for cultivars or strain identification and classification of H.compressa are also discussed.
【Fund】: 重庆市自然科学基金(CSTC 2006BA1022);; 教育部“新世纪优秀人才支持计划”(NCET-04-0909)资助
【CateGory Index】: S543.5
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