Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Prataculturae Sinica》 2008-01
Add to Favorite Get Latest Update

Research on a transformation acceptor system of Cichoriun intybus mediated by Agrobacterium tumefaciens

CHENG Lin-mei1,SUN Yi1,WANG Yi-xue1,WU Jia-he2(1.Shanxi Agri-Biotechnology Research Center,Taiyuan 030031,China;2.Institute of Cotton Research,Shanxi Academy of Agricultural Science,Yuncheng 044000,China)  
Several important influential factors on a transformation acceptor system of Cichoriun intybus were studied and it was shown that the transformation frequency was 100%,when the leaves and caudexes as explants were cultivated on a co-culture medium of MS+6-BA 1.5 mg/L+IBA 0.2 mg/L.Furthermore,the genetic transformation of leaves for 20-25 d after rhizogenesis was most effective using the genetic transformation system of C.intybus mediated by Agrobocterium tumefaciens LBA4404.When the C.intybus leaves were co-cultured for 2 d with added ranamycim(20-25 mg/L) and cefotaxime(500-600 mg/L),the transformation frequency increased from 1.5% to about 7.1%.
【Fund】: 山西省自然科学基金项目(No.200601192);; 山西省农业科学院高新项目(No.YGX0507)资助
【CateGory Index】: S548
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 ZHAO Yu-wei1,2,WANG Ying-juan1,BU Huai-yu1,HAO Jian-guo1,JIA Jing-fen1(1.College of Life Science,Northwest University,Xi'an 710069,China;2.State Key Laboratory of Transient Optics and Photonics,Xi'an 710069,China);Transformation of Cichorium intybus with the AtNHX1 gene and salinity tolerance of the transformants[J];Acta Prataculturae Sinica;2009-03
Chinese Journal Full-text Database 8 Hits
1 ZHOU Si jun 1,LI Xi chen 1,LIU Zhao jun 1, LIU Li yan 1 and YANG Qing kai 2 (1 Biotechnology Research Center,Heilongjiang Academy of Agricultural Sciences,Harbin 150086; 2 Institute of Soybean,Northeast Agricultural University,Harbin 150030;Optimization of the Agrobacterium-mediated transformation systems of soybean[J];Journal of Northeast Agricultural University;2001-04
2 CHENG Lin-mei~1, CAO Qiu-fen~(1), GAO Hong-wen~(2*), HUANG Jing~1, MENG Yu-pin~1 (1.Shanxi Agri-Biotechnolgy Research Center, Taiyuan, Shanxi Province 030031, China; 2.Research and Development Center for Grassland Science, China Academy of Agriculture Sciences, Beijing, 10081, China);Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)[J];Acta Agrestia Sinica;2004-03
3 ZHANG Zhenxia1, CHU Chengcai2, FU Yikun1(1.Gansu Agricultural University, Lanzhou 730070, China; 2. Institute of Genetics and Developmental Biology, Chinese Academyof Sciences, Beijing 100101, China);<title>Transformation of Leguminous forage Lotus corniculatus by Agrobacterium mediated GA 20oxidase gene[J];Acta Pratacultural Science;2002-03
4 HUANG Yong-hong,YI Gan-jun~*,ZHOU Bi-rong,ZENG Ji-wu,WU Yuan-li(Fruit Research Institute,Guangdong Academy of Agriculture Science,Guangzhou 510640,China);Research Advances about Genetic Engineering of Banana[J];Acta Botanica Boreali-Occidentalia Sinica;2006-10
5 GU Jun-tao1,2,ZHAO Hong-mei1,3,LIU Zhu-ling1,XIAO Kai1(1.College of Agronomy,Baoding 071001,China;2.College of Life Science,Baoding 071001,China;3.College of Information Science and Technology Agricultural University of Hebei,Baoding 071001,China);Genetic transformation of Trifolium repens using Agrobacterium tumefaciens and transgenic plant regeneration with high efficiency[J];Acta Prataculturae Sinica;2007-02
6 ZHANG Zhen-xia~(1,3), YANG Zhong-yi~1, CHU Cheng-cai~2logical Control, Guangzhou 510275, China; 2. Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, Beijing 100101, China; 3.Department of Biology, Hanshan Normal college, Chaozhou 521041, China);Progress in transgenic research of gramineous forage and turfgrass plants[J];Acta Pratacultural Science;2004-06
7 Zhou Jiming Wei Zhimfng Xu Zhihong Liu Shigui Luo Peng (National Laboratory of Plant Molecolar Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032) (Department of Biotechnology and Department of Biology of Sichuan University, Chendu 610064);Agrobacterium-mediated transformation of Orychophragmus Violaceus Cotyledon and Regeneration of Transgenic Plants[J];Chinese Journal of Biotechnology;1996-01
8 Lan Haiyan Chen Zhenghua Institute of Genetics,Chinese Academy of Sciences,Beijing 100101;Advances in Plant Genetic Transformation Techniques of Brassica by Agrobacterium-mediated Method[J];BIOTECHNOLOGY INFORMATION;1999-03
Chinese Journal Full-text Database 10 Hits
1 TAO Jing~(1,2),CHEN Shi-gang~2,LIU Bao~1,LI Tie~3,LI Qing-mei~2,ZHANG Wei-sheng~~2,WANG Xian-cheng~2(1.School of Life Sciences,Northeast Normal University,Changchun 130024,China;2.Jilin Provincial Academy of Forestry Science,Changchun 130033,China;3.Sanchazi Forestry Bureau of Jilin Province,Baishan 134702,China);The introduction and resistance evaluation of Elaeagnus commutata[J];Journal of Northeast Normal University;2006-01
2 SU Shaokun,LIU Hongyu,QIN Zhiwei(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Establishment of genetic transformation system by Agrobacterium-mediated parthenocarpy gene of cucumber[J];Journal of Northeast Agricultural University;2006-03
3 HUANG Yu-ji1,2,LAI Zhong-xiong1,ZHAO Qiao-yang1(1.Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Institute of Fruits,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China);Effect of silver nitrate on genetic transformation in banana(Musa spp.)[J];Subtropical Agriculture Research;2008-01
4 DING Cheng-long~(1,2),SHEN Yi-xin~(1),GU Hong-ru~2,Yuichi Miura~3(1.College of Animal Science and Technology,Nanjing Agriculture University,Nanjing,Jiangsu Province 210095,China;2.Institute of Animal Science,Jiangsu Academy of Agricultural Science,Nanjing,Jiangsu Province 210014,China;3.Institute of Forage Crop,Japan Grassland Farming and Forage Seed Association,Tochigi 329-2742,Japan);Identification of an EST-CAPS Marker Linking to the Resistance Gene of Italian Ryegrass Gray-Leaf-Spot Disease by Bulked Segregant Analysis[J];Acta Agrestia Sinica;2006-01
5 CUI Guo-wen(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China);Perspective,present and tasks in forage breeding in China[J];Pratacultural Science;2008-01
6 DONG She-qin et al(College of Life sciences,Yangtze University,Jingzhou,Hubei 434025);Effects of Natural Extracts on Callus Induction of Davidia involucrata Baill Stem[J];Journal of Anhui Agricultural Sciences;2007-29
7 CUI Gui-mei1,YANG Li-li2,SUN Yi1,ZHANG Yan-qin2(1 The Agri-Biotechnology Research Center of Shanxi Province,Taiyuan 030031,China;2 Dry Land Agriculture Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China);Correlation Study between Bud Length and Gametophyte Development Stage in Daylily(Hemerocallis hybridus cv.'Stella de oro')[J];Acta Botanica Boreali-Occidentalia Sinica;2008-10
8 WANG Dong-mei~1,TANG Yan-qiong~2,ZHUO Peng~1 (1.State Key Laboratory of Tropical Crop Biotechnology,Institute of Tropical Science,Academy of Tropical Agricultural Science,Haikou 571101,China;2.Agricultural College,The South China University of Agriculture,Danzhou 571737,China);An investigation of approaches to optimize gene transformation system of Stylosanthes guianensis ReyanⅡ[J];Pratacultural Science;2005-10
9 Wang Xiaochun 1 Liu Shangqian 1 Ji Jing 2 Wang Ping 2 (1 Department of Agronomy,Hebei North University, Xuanhua 075131; 2 Changchun University of Agricultural and Animal Science,Changchun 100062);THE AFFECT FACTORS ON THE GERMINATION FREQUENCY OF SOMATIC EMBRYOS OF SOYBEAN[J];Soybean Science;2004-02
10 Wang Xiaochun 1,2,3 Liu Shangqian 3 Ji Jing 1,2 Wang Gang 1,2 Wang Ping 2 (1.Tianjin University of Agricultural and Biological Engineering,Tian jin; 2.Changchun University of Agricultural and Animal Sciences, Changchun 130062; 3.Department of Agronomy,HeBei North University, Xuanhua 075131);OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROBACTERIUM TUMEFACIENS[J];Soybean Science;2004-03
China Proceedings of conference Full-text Database 1 Hits
1 Yang guangdong, Li yan'e. Shangguan Xiaoxia (Cotton research institute, Shanxi academy of agricultural science, Yuncheng 044000);Study on the Optimization of Transformation System in Chinese Cabbage[A];[C];2000
Chinese Journal Full-text Database 8 Hits
1 Song Shufeng1 Cao Feng2 Yang Peizhi3 Chen Mingli2 Li Chuanshan2 Guo Aiguang1,2* 1 College of Life Science, Northwest A&F University, Yangling, 712100; 2 Shaanxi Key Laboratory of Agriculture Molecular Biology, Yangling,712100; 3 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100;;High Efficient System Establishment on Plant Regeneration and Study on Genetics Transformation in Puna Chicory (Cichorium Intybus L.)[J];Molecular Plant Breeding;2006-04
2 XU Gui-fang(Changsha Vocational College of Environmental Protection,Changsha 410004,China);Effects of PEG stress on resistance physiological and biochemical indexes of adversity of two Lysimachia species[J];Acta Prataculturae Sinica;2008-01
3 XUE Yan-feng,LIU Zhao-pu (College of Resources and Environmental Science,Nanjing Agricultural University,Nanjing 210095,China);Effect of exogenous calcium on alleviation of the decreased photosynthetic ability of Helianthus tuberosus leaves under seawater stress[J];Acta Prataculturae Sinica;2007-06
4 ZHAO Yu Wei1,2,3 BU Huai Yu1,2,3 HAO Jian Gao1,2,3 WANG Ying Juan1,2,3 JIA Jing Fen1,2,3(1College of Life Science,Northwest University;2Provincial Key Laboratory of Biotechnology;3Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education Northwest University,Xi′an 710069);TRANSFORMATION OF ASTRAGALUS MELILOTOIDES PALL WITH AtNHX1 GENE AND THE EXPRESSION OF SALINITY TOLERANCE OF TRANSFORMANTS[J];Journal of Molecular Cell Biology;2008-03
5 XUE Zhe yong 1, ZHI Da ying 1, XIA Guang min 1 MA Xiu ling 2, ZHANG Hui 2 & ZHAO Yan xiu 2 (1.School of Life Science, Shandong Univ., Jinan 250100; 2.Laboratory of Plant Abiotic Stress Tolerance, Shandong Normal Univ., Jinan 250014);Agrobacterium Tumefaciens Mediated AtNHX1 Gene Transformation to Wheat[J];Journal of Shandong University(Natural Science);2003-01
6 MAO Gui-Lian, XU Xing (Agricultural Bio-technological Lab, Ningxia Academy of Agriculture and Forestry, Yinchuan 750002), XU Zhao-Zhen (Agricultural Department of Ningxia Agricultural College, Yongning 750105);Advances in physiological and biochemical research of salt tolerance in plant[J];Chinese Journal of Eco-Agriculture;2004-01
7 LIU Jing1,2, ZHOU Shu-feng1, CHEN Hua1, HAN He-ping1, LI Yin-xin1 (1 Key Laboratory of Phytosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093; 2 The Graduate School of Chinese Academy of Sciences, Beijing 100039);Agrobacterium Mediated Transformation of BADH to the AtNHX1 Transgenic Tomato (Lycopersicum esculentum L. ‘Moneymaker’)[J];Scientia Agricultura Sinica;2005-08
8 WANG Yan~(1,2),ZENG You-Ling~1,HE Bin~2,QIN Li~1,LI Jin-Yao~1,GAO Yan~2 and ZHANG Fu-Chun~(1,)(~1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,Xinjiang;~2 Institute of Industrial Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830000,Xinjiang,China);Research of NHX Gene Transformation in Brassica napus by Agrobacterium tumefaciens[J];Acta Agronomica Sinica;2006-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MA Zhong hua, ZHANG Yun fang, XU Chuan xiang,CHEN Wen Jun, YING Hong hua, KUAI Ben ke (Department of Biochemistry);Tissue culture and Genetic Transformation of Kentucky Bluegrass ( Poa pratensis) via Microprojectile Bombardment[J];JOURNAL OF FUDAN UNIVERSITY;1999-05
2 Liu Qingfa; Tang Kexuan; Ye Jianming; Hao Zhengrong; He Yiyuan; Shen daleng(Institute of Genetics, Fudan University);Optimization of the Conditions for Wheat Genetic Transfermation Mediated by Agrobacterium Tumefaciens[J];JOURNAL OF FUDAN UNIVERSITY;1998-04
3 GUO Zhen-fei,LU Shao-yun (Laboratory for Turfgrass Science,College of Life Science,South China Agricultural University,Guangzhou 510642,China);Application of Genetic Engineering in Turfgrass Breeding[J];Acta Agrestia Sinica;2002-03
4 Hu Dixian;Feng Chaobi; Zhu Bangchang(Quizhou College of Agriculture,Guiyang,Guizhou 550025);The Study on Nutrition Development of White Clover[J];ACTA PRATACULTURAL SCIENCE;1994-02
5 LI Yu tang (China Prataculture Association, Beijing 100094);Strategy for environmental management and economical development of North west China and Yellow River region[J];ACTA PRATACULTURAL SCIENCE;2000-01
6 CHEN Zhiyong,YI Zili(Laboratory of Cell Engineering, Hunan Agricultural University, Changsha 410128, China);The research of improving the inductivity of callus of Festuca arundinacea[J];Acta Pratacultural Science;2003-04
8 CHEN Chen1, CAO Zhi-zhong1, HE Shun-ji2, QIAO Dai-rong2, CAO Yi1 (1. College of Pratacultural Science, Gansu Agricultural University, Gansu, Lanzhou 730070; 2. College of Biology, Sichuan University, Sichuan, Chendu 610065, China);Establishment and optimization of Agrobacterium-mediated transformation of alfalfa (Medicago sativa L.)[J];Journal of Gansu Agricultural University;2004-05
9 LI Ya 1,2 , LIU Jian xiu 2, XIANG Qi bo 1 (1.Nanjing Forest University,Nanjing 210037, China; 2.Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China);Progress in zoysiagrasses germplasm resources research[J];Acta Pratacultural Science;2002-02
10 Li Huaping;John S Hu;Kelly Barry; Fan Huaizhong(South China Agricultural University, Guangzhou 510642)The plant expression vectors of the CP genes of Cucumber mosaic virus (CMV)strains in -ecting banana were constructed, and were used to transform;STUDIES ON TRANSGENIC TOBACCO PLANTS WITH THE COAT PROTEIN GENE OF CUCUMBER MOSAIC VIRUS STRAINS INFECTING BANANA[J];Chinese Journal of Virology;1996-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved