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《Acta Prataculturae Sinica》 2008-03
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Effect of exogenous hormone on callus induction and chromosome doubling of Glycyrrhiza uralensis

MA Wen-fang1,HUANG Hui-ying1,WANG Qing2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)  
Various explants of Glycyrrhiza uralensis(cotyledons,hypocotyls,and radicles) were used to study the the concentration effects of the exogenous hormone NAA,of 2,4-D and BAP on callus induction,bud regeneration and chromosome doubling.There was no significant difference in frequency between the different types of explants for callus induction and the MS+1.0 mg/L NAA+1.0 mg/L 2,4-D+1.0 mg/L BAP medium induced the highest efficiency(up to 100%).Regeneration from hypocotyls was the best,and with MS+3.0 mg/L BAP +2.0 mg/L ZT+10.0 mg/L GA3 medium,the highest frequency of regeneration reached 4.57%.Chromosome ploidy was also affected by type of explants and by concentration of NAA and 2,4-D.The frequency of hypocotyl tetraploids was significantly higher than that of cotyledons and radicles,with an average of 42.23%.Using 1.0 mg/L 2,4-D,the frequency of tetraploids of cotyledons,hypocotyls and radicles reached levels of 61.25%,60.00% and 58.13% respectively.Furthermore,the frequency of chromosome doubling increased with prolonged callus culturing time.
【Fund】: 甘肃省自然基金项目(3ZS041-A25-047)资助
【CateGory Index】: S567.71
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