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《Acta Prataculturae Sinica》 2009-01
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An analysis by ISSR of genetic diversities in Stylosanthes germplasms

TANG Yan-qiong1,HU Xin-wen1,GUO Jian-chun2,BAI Chang-jun3,HE Hua-xuan3(1.College of Agriculture,Hainan University,Danzhou 571737,China;2.Institute of Biotechnology and Bioscience,CATAS,Haikou 571101,China;3.Institute of Tropical Crops Genetic Resources,CATAS,Danzhou 571737,China)  
Forty-eight Stylosanthes germplasms were studied for an analysis of genetic diversity by ISSR.Fourteen effective primers selected from 68 ISSR primers were used for PCR,and 155 of the 156 DNA fragments amplified,showed polymorphisms.The average binds from each primer was 11.1 and the average percentage of polymorphic bands was 99.36%.The coefficient of genetic conformability among the germplasms was 0.445-0.975.The results from POPGENE analysis indicated that the average information index(I) of Shannon was 0.355 3,gene diversity(H) of Nei was 0.227 9,and effective number of alleles(NE) was 1.388 7.Based on analyses of cluster and principal components,the 48 Stylosanthes germplasms used in this study could be divided into 5 groups: S.hamata,S.capitata,S.scabra,S.seabrana and S.guianensis,in which the genetic differentiation of S.guianensis group was the largest.
【Fund】: 国家重点基础研究发展计划(973计划)(2007CB108903);; 作物遗传育种国家重点学科和海南省自然科学基金项目资助
【CateGory Index】: S541.9
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