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《Acta Prataculturae Sinica》 2009-03
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Genetic diversity identification based on ISSR analysis of Agrostis stolonifera germplasm resources in Guizhou

SHANG Yi-shun1,YANG Chun-yan2,ZHONG Li1(1.Guizhou Prataculturale Institute,Dushan 558200,China;2.Agricultural Bureau of Duyun,Duyun 558000,China)  
Agrostis stolonifera is a widely grown,cool season turf grass in China.To determine genetic diversity and relationships of A.stolonifera resources,285 plants from three wild populations and a cultivated population in Guizhou were screened by inter-simple sequence repeat(ISSR)markers.Nine of 60 primer pairs produced a total of 81 bands,of which 66 were polymorphic.Statistic analysis showed a rich genetic diversity of A.stolonifera resources in Guizhou province.The genetic differentiation coefficient(Gst) and the gene flow(Nm)among three wild populations were 0.492 0 and 0.516 4.The genetic differentiation was considerable in the three wild populations.The genetic distance GD)of the subpopulations ranged from 0.087 7 to 0.592 6.The subpopulations could be clustered into three groups based on GD.Correlation analysis found a significant positive correlation between geographical distance and genetic distance(r=0.494),and this is persuasive evidence that there is a significant correlation between genetic component and the geographic distribution of A.stolonifera resources.Further genetic relationship analysis discovered a high genetic similarity among wild populations,ranged from 0.700 3 to 0.940 9 while the cultivated population(KROMI) had an obvious genetic differentiation from wild populations.
【Fund】: 贵州省草业工程技术中心建设项目[黔科合农字(2005)4002号];; 贵州省省长基金项目[合同号:黔省专合字(2006)87号];; 贵州省“十一五”攻关项目[黔科合NY字(2008)3047号]资助
【CateGory Index】: S688.4
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