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《Acta Prataculturae Sinica》 2009-03
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Protoplast culture and plant regeneration of Zygophyllum xanthoxylum

WANG Ying-hua1,2,CHEN Gang2,JIA Jing-fen1,HAO Jian-guo1(1.Provincial Key Laboratory of Biotechnology,Northwest University,Xi'an 710069,China;2.Institute of Life Science,Zhaoqing Uiversity,Zhaoqing 526061,China)  
An efficient protocol for plant regeneration from protoplasts of Zygophyllum xanthoxylum was established.The friable calli induced from segments of cotyledon were used for protoplast isolation through enzyme digestion.The effects of different media and plating densities on protoplast divisions and plant regeneration were studied.Sustained cell divisions and colony formation from the protoplasts of Z.xanthoxylum were obtained by a DPD medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D),0.2 mg/L 6-benzylaminopurine(6-BA),0.2 mg/L kinetin(KT),0.5 mol/L mannitol,500 mg/L casein hydrolysate,and 2%(W/V) sucrose at a plating density of 2×105 /mL.The division frequency was 28.4%.Regenerated plantlets were obtained when these calli were transferred to MS medium containing 1.0 mg/L 6-BA and 0.5 mg/L(NAA).
【Fund】: 国家自然科学基金项目(30671082)资助
【CateGory Index】: S567.239;S548
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