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《Acta Prataculturae Sinica》 2009-06
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A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene

YAN Li-ping1,XIA Yang1,LIANG Hui-min2,WANG Zhen-zhen1,LI Shuang-yun1,LIU Cui-lan1,PANG Cai-hong1(1.Shandong Provincial Academy of Forestry,Jinan 250014,China;2.Jiangsu Agriculture and Forestry Profession Technology College,Jurong 212400,China)  
The physiological response of transgenic alfalfa to different salt stresses and its PCR detection were investigated.The ratio of negative to positive numbers of PCR detection confirmed the first Mendelian law.The transgenenic T1 generations showed enhanced BADH activity and betaine content,compared to the non-transgenenic plants at 0.8% NaCl stress.Proline and soluble sugar contents and the antioxidase activity of transgenic T1 generations were higher than those of non-transgenic plants at different salt stresses,while electrolyte leakage and malonaldehyde contents were lower.This suggests that the BADH gene was expressed in the transgenic T1 generation,resulting in improved salt tolerance in the transgenic plants.
【Fund】: 山东省良种工程项目(2006lz12-02)资助
【CateGory Index】: S54
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