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《Acta Prataculturae Sinica》 2010-05
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Effects of NaCl stress on proline metabolism of Achnatherum splendens seedling

DONG Qiu-li,XIA Fang-shan,DONG Kuan-hu (College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China)  
Under varying levels of NaCl stress,the proline(Pro),pyrroline-5-carboxylate synthetase(P5CS),ornithine-oxo-acid transaminase(δ-OAT),and proline dehydragenase(ProDH)among leaves and roots of Achnatherum splendens were determined.The results showed that the proline metabolism synthesis path-way of the A.splendens seedling is mainly on the pathway of Glutamate.The proline content increased with the increase of NaCl,and the proline content in leaves and in roots were the highest that could be up to 585.77and 303.36μg/g FW respectively at the concentration of 500mmol/L NaCl.The activity of δ-OAT was significantly lower than that in control(P0.05),and declined with the increasing of the NaCl concentration.The activity of P5CS from the roots of Achnatherum splendens was significantly higher than leaves under the same concentration(P0.05).At the concentration of 300mmol/L NaCl,the P5CS activity in leaves and roots reached the 7.44and 20.81U/mg Protein respectively.The activity of ProDH in leaves were higher than in control,and could be up to 1.7times than the control,but didn't change significantly in roots.
【Fund】: 国家“十一五”科技支撑计划课题(2007BAD56B01)资助
【CateGory Index】: S543.903.4
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