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《Acta Prataculturae Sinica》 2010-05
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Genetic diversity in white clover germplasm detected by SRAP markers

ZHANG Jing-yuan,PENG Yan,LUO Yan,MA Xiao (Department of Grassland,Sichuan Agricultural University,Ya’an 625014,China)  
SRAP(sequence-related amplified polymorphism)markers were used to analyze genetic diversity of 41 white clover(Trifolium repens)accessions using 20 pairs of random primers.The results were:1)The primer pairs amplified 479clear bands,including 411 polymorphic bands,which ranged from 13 to 44 bands per primer pair with an average of 23.95 bands.The percent of polymorphic loci was 85.16%,and the polymorphism information content ranged from 0.182to 0.334 with an average of 0.268,which showed that the tested white clover had a rich genetic diversity of germplasm.2)Genetic similarity ranged from 0.586 6to 0.989 6 among all accessions with an average of 0.730 7,which also suggested that there was a rich genetic variation among the white clover germplasms.3)Cluster analysis and principal component analysis of 41white clover accessions divided them into 3 groups on the genetic similarity of 0.71 and most of the accessions from the same or similar ecologic geographical environment clustered in one group,showing a certain degree of eco-geographical distribution.
【Fund】: 国家科技支撑计划(2008BADB3B10);; 四川农业大学青年创新基金(002325)资助
【CateGory Index】: S541.2
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