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Genetic diversity analysis of Chinese natural tall fescue(Festuca arundinacea) germplasms by RAPD

LI Hui-ying,LOU Yan-hong,HU Tao,FU Jin-min(Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,CAS,Wuhan 430074,China)  
Tall fescue(Festuca arundinacea) is a cool season turfgrass widely used in China.The genetic variation of wild tall fescue germplams was investigated.Twenty six tall fescue ecotypes collected from Yunnan,Guizhou,Qinghai and Hubei provinces were assessed for genetic diversity analysis by random amplified polymorphic DNA(RAPD).Twenty of the 41 arbitrary primers totally amplified 179 DNA bands,an average of 8.95 DNA bands per primer.The polymorphic bands(177) accounted for 98.88% of total DNA bands.Jaccard's genetic similarity coefficient of these tall fescue ecotypes ranged from 0.419 to 0.966,suggesting a significant variation among these ecotypes.An UPGMA dendrogram was established for 26 tall fescue ecotypes based on the genetic similarity matrix.The 26 ecotypes could be divided into three categories based on cluster analysis.The tall fescue ecotypes collected from Yunnan province located in one group.However,tall fescues that came from the other three provinces were not always closely related to their ecological environment.
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