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《Acta Prataculturae Sinica》 2011-01
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SSR analysis of the linkage drag of endosperm mutation gene ae

QIAO Yan1,WANG Han-ning2,ZHANG Cheng1,YANG Fang1(1.Agronomy and Forestry Science Department of Longdong University,Qingyang 745000,China; 2.Agronomy College,Gansu Agricultural University,Lanzhou 730070,China)  
Molecular assisted selection was used to analyse linkage drag of different recurrent parents on two flanks of two populations and for restoring the ratio of two populations by SSR markers for ae.These are evenly distributed on chromosome 5.Linkage drags at the two flanks were controlled in 13.2 and 17.2 cM of CHBC1F2 populations by SSR markers and the linkage drag at one flank was controlled in 13.9 cM of DHBC1F2 populations.Genetic background resemblance of individuals of the CHBC1F2 population to the recurrent parent's was 85.7%.Genetic background resemblance of individuals of the DHBC1F2 population to the recurrent parent's was 87.5%.It is possible to identify recombination individuals with the targeted gene through a high density(10-20 cM per marker) map.This reduced unnecessary fragmentation of chromosomes and eliminated linkage drag of the targeted gene in backcross breeding.The hitchhiking effect may have been reduced.
【Fund】: 国家高技术研究发展计划“863”计划项目(2004AA207140)资助
【CateGory Index】: S513
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