Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Prataculturae Sinica》 2011-02
Add to Favorite Get Latest Update

Establishment of an Agrobacterium mediated genetic transformation system for Eremochloa ophiuroides

ZHANG Fang,WANG Zhou,ZONG Jun-qin,LIU Jian-xiu,SHE Jian-ming(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China)  
Agrobacterium tumefaciens strain LBA4404 harboring the plasmid pCAMBIA1301 which contains stress tolerance-related transcription factor ZjDREB1 gene and reporter gene HPT was used to transform axillary bud-derived embryogenic calluses of Eremochloa ophiuroides 'E126'.To systematically optimize the conditions of centipedegrass transformation,several factors which could influence Agrobacterium-mediated DNA transfer were examined,including concentration of selective agent and bacteriostat,bacterial concentration,duration of infection and co-cultivation.Consequently,a transformation system of transgenic centipedegrass plants was established.It was found that the efficiency of transformation highly relied on the following optimal conditions: The concentration of hygromycin for selection was 30 mg/L;the cefotaxime concentration for restraining the Agrobacterium was 400 mg/L;OD600 as 0.3,30 min of infection time and 3 days.Co-cultivation time was the optimized condition for genetic transformation system in E.ophiuroides.Ten transgenic plants were identified by PCR detection from initial 44 regenerated plants.
【Fund】: 江苏省科技支撑项目(BE2008403);; 江苏省科技公共服务平台项目(BM2009905)资助
【CateGory Index】: S688.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 SHE Jian-ming~1, ZHANG Bao-long~1, HE Xiao-lan~1, CHEN Zhi-yi~2, NI Wan-chao~1 (1.Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China; 2.Institute of Forage Industry, Zhejiang Shaoxing Baiyun Construction Co. Ltd, Xinchang, Zhejiang Province 312500, China);A Study on the Conditions of Genetic Transformation into Poa pratensis L. by Agrobacterium Tumefaciens[J];Acta Agrestia Sinica;2005-01
2 LIU Yan-xiang,DONG Kuan-hu (College of Animal Science and Technology,Shanxi Agriculture University,Taigu 030801,China);Transcription factor CBF and its cold tolerance mechanism[J];Pratacultural Science;2009-05
3 Ren Jian Mao Kai Fan Yan (Sichuan Agricultural University,Yaan 625014);ADAPTABILITY OF COMMON CENTIPEDEGRASS[J];PRATACULTURAL SCIENCE;1998-05
4 MA Hui-ling~1,LU Xin-shi~2,CAO Zhi-zhong~1,YU Mi-mi~2(1.Pratacultural College Gansu Agricultural University,Lanzhou 730070,China;2.Resources and Environment College of Beijing Foresty University,Beijing 100083,China);Analysis of factors influencing gene transformation in Medicago sativa[J];Acta Prataculturae Sinica;2006-05
5 YUAN Xue-jun1,2,WANG Zhi-yong1,GUO Ai-gui1,ZONG Jun-qin1,LIU Jian-xiu1,SHE Jian-ming1 (1.Grass Research Centre,Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing 210014,China;2.College of Life Science,Nanjing Agricultural University,Nanjing 210095,China);Callus induction and plantlet regeneration from axillary buds of Eremochloa ophiuroides[J];Acta Prataculturae Sinica;2008-06
6 ZHAO Yu-wei1,2,WANG Ying-juan1,BU Huai-yu1,HAO Jian-guo1,JIA Jing-fen1(1.College of Life Science,Northwest University,Xi'an 710069,China;2.State Key Laboratory of Transient Optics and Photonics,Xi'an 710069,China);Transformation of Cichorium intybus with the AtNHX1 gene and salinity tolerance of the transformants[J];Acta Prataculturae Sinica;2009-03
7 ZHANG Gai-na1,2,JIA Jing-fen2(1.College of Agriculture of Henan University of Science and Technology,Luoyang 471000,China;2.School of Life Science,Northwest University,Xi'an 710069,China);Cloning of PA1 gene from Pisum sativum and genetic transformation on Medicago sativa[J];Acta Prataculturae Sinica;2009-03
8 YANG Feng-ping~1,LIANG Rong-qi~1,ZHANG Li-quan~1,ZHANG Xiao-dong~(1*),SUN Zhen-yuan~2(1 Beijing Agro-Biotechnology Research Center,Beijing Agriculture and Forestry Academy,Beijing 100089,China;2 Institute of Forestry Science,Chinese Academy of Forestry,Beijing 100091,China);Perennial Ryegrass Transformed with the Adversity-Resistant Transcription Factor DREB1B Gene[J];Acta Botanica Boreali-Occidentalia Sinica;2006-07
9 LIU Zhi xue, MA Xiang qian, HE Yi yuan,Xu Ya nan, YE Ming ming, TANG Ke xuan (Institute of Genetics);An Improved Assisting Method for Genetic Trans formation via Agrobacterium tumefaciens[J];JOURNAL OF FUDAN UNIVERSITY;1999-05
10 Lv Yan1 Wang Pingrong2 Sun Yeying2 Dong Chunlin2 Chen Dexi2 Deng Xiaojian2*1 College of Chemistry Biology and Technology, Yantai University, Yantai, 264005; 2 Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130;Application of Agrobacterium-mediated Transformation in Rice Genetic Engineering[J];Molecular Plant Breeding;2005-04
Chinese Journal Full-text Database 10 Hits
1 HE Yan1,ZANG De-kui2,CUI Lin3,ZANG Zhen-rong4(1.Landscape Bureau of Shizhong District of Jinan City,Jinan 250002,China;2.Forestry College,Shandong Agricultural University,Taian 271018,China;3.Forestry Center of Jinan City,Jinan 250002,China;4.Shandong Academy of Forestry Science,Jinan 250014,China);Investigation on Woody Ground Cover Plants in Jinan City[J];Shandong Agricultural Sciences;2008-04
2 HONG Xiao-xiao,YU Xiao-ying(College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China);Research Progress in Drought Resistance Mechanism of Ground Cover Plants[J];Shandong Agricultural Sciences;2009-05
3 Zhang Kai et al (Educational Depatment of Luan City,Luan 237001);Influence of some Different Micro-fertilizer on Physiological Characteristics of Pony foot[J];Journal of Anhui Agricultural Sciences;2003-03
4 WANG Chang-teng et al(Lishui Vocational College,Lishui,Zhejiang 323000);Application of Native Plant Resources of Lishui in Landscape[J];Journal of Anhui Agricultural Sciences;2006-04
5 WU Jin-dong et al (Biochemistry Department, Western Anhui University, Luan, Anhui 237012);Study on the Optimum Management Mode of Tall Fescue Lawn[J];Journal of Anhui Agricultural Sciences;2006-08
6 GAO Xiang-bin et al(College of Agronomy,Liaocheng University,Liaocheng,Shandong 252059);Application of Allelonathy in the Construction of City Ecological Garden[J];Journal of Anhui Agricultural Sciences;2006-23
7 XING Yu-jun et al(Departerment of Biology,Huaiyin Teachers College,Huaian,Jiangsu 223300);Research Progress in Genetic Transformation of Sweet Potato Mediated by Agrobacterium tumefaciens[J];Journal of Anhui Agricultural Sciences;2007-24
8 DING Li et al(College of Agronnomy,Hainan University,Danzhou,Hainan 571737);Study on Application of Lawn in Landscape Design[J];Journal of Anhui Agricultural Sciences;2009-09
9 DING Sheng et al(The Management of Shushan Forest Park,Hefei,Anhui 230036);Application Investigation of Ground Cover in Hefei City Green Land[J];Journal of Anhui Agricultural Sciences;2009-12
10 LIAO Li et al (College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Effect of Low Temperature on Osmotic Aadjustment Substances in the Leaves of Eremochloa ophiuroides(Munro.)Hack.[J];Journal of Anhui Agricultural Sciences;2009-21
China Proceedings of conference Full-text Database 7 Hits
1 JIN Xiao-ling WANG Xiao-hong SHEN Shou-yun JLANG Li (School of Eniviroment Art Design,Central South Univenity of Forestry and Technology,Changsha 410004);The Investation of Colorful Plant Species and Their Application in Changsha[A];[C];2007
2 ;Study on the Adaptability of Tall Fescue and Kentucky Bluegrass Cultivars or Lines in Shanghai[A];[C];2008
3 LIU Jin-ping,YOU Ming-hong,MAO Kai,BAI Shi-qie,TANG Xue-hui (Department Of Grassland Science, Sichuan Agricultural University,Yaan 625014,China Sichuan Institute of Grassland , Chengdu 611731,China);The Influence of Potassium on Population of Centipedegrass[A];[C];2004
4 LIU Lin, MAO Kai, BAI Shi-qie, GAN You-min, ZHANG Xin-quan, YANG Chun-hua, TANG Xue-hui (Sichuan Agricultural University, Ya'an 625014 China; Sichuan Grassland Research Institute, Chengdu 611731 China);Study on Enhancing Cold Resistance of Sichuan Wild Centipedegrass by Way of Cold Hardening[A];[C];2004
5 Gao He Liu Jianxiu Ma Kequn (Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014;Nanjing Agricultural University, Nanjing 210095);Research and Use of Ornamental Grasses[A];[C];2006
6 Bai Shiqie~1 Yan Jiajun~(1,2) (1.The Department of Grassland Science,Sichuan Angricultural University,Ya'an 625014; 2.Sichuan Academy of Grassland Science.Chengdu 611731);Study Progress in Germplasm Resources of Eremochloa ophiuroides(Munro.)Hack.[A];[C];2007
7 ;The Light Response of Turfgrass[A];[C];1996
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Bao Weibao;Han Liebao;Yin Shuxia. Journal of Beijing Forestry University (2000)22(2)71~73[Ch,2 ref.] Forestry Farm of Zhoushan City,Zhejiang Province,316000,P.R.China.;Adaptation research on perennial ryegrass varieties in Beijing.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-02
2 Shen Xiaohui; Lu Hai; Wang Shasheng; Jiang Xiangning; Yill Sung Park; M. K. Mahendrappa. School of Biological Science and Biotechnology, Beijing For. Univ., 100083, P. R. China.;Molecular cloning and expression of Choline Monooxygenase(CMO) gene in Escherichia coli.[J];Journal of Beijing Forestry University;2003-01
3 Wang Cheng; Li Qing; Xin Yan. School of Landscape Architectur e, Beijing For. Univ.,100083, P.R.China.;Callus induction and plant regeneration from seeds of Festuca arundinacea[J];Journal of Beijing Forestry University;2004-01
4 QI Chun-hui;HAN Lie-bao;LIANG Xiao-hong;ZENG Hui-ming;LIU Jun.Key Laboratory for Silviculture and Conservation,Ministry of Education,Institute of Turfgrass Science,Beijing Forestry University,100083,P.R.China.;Transgenic Zoysia japonica plants obtained by biolistic bombardment transformation.[J];Journal of Beijing Forestry University;2006-03
5 SHE Jian-ming 1, ZHANG Bao-long 1, CHEN Zhi-yi 2, NI Wan-chao 1 (1.Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China; 2.Zhejiang Shaoxing Baiyun Construction Co. Ltd, Xinchang, Zhejiang Province 312500, China);A Study on the Technique of Plant Regeneration from Mature Seed Embryo of Kentucky bluegrass in vitro[J];Acta Agrestia Sinica;2003-01
6 ZHANG Wanjun, WANG Tao(National Laboratory for Agriculture and Biotechnology, China Agriculture University, Beijing, China 100094);A Study on the Tissue Culture and Plant Regeneration of Perennial Ryegrass[J];Acta Agrestia Sinica;2003-03
7 ZHANG Zhen-xia~1, CHU Cheng-cai~2,XI Jia-bin~1,Chen Ping~3(1.School of Life Science, Sun Yat-sen University, Guangzhou, Guangdong Province 510275, China; 2.Department of Biology, HanShan Normal College, Chaozhou,Guangdong Province, 521041,China; 3.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;4. Department of Agronomy, Zhongkai Agrotechnical College, Guangzhou,Guangdong Province, 510225, China);Embryogenic Callus Production and Plant Regeneration of Lolium Perenne Seed Explants for Genetic Manipulation[J];Acta Agrestia Sinica;2004-04
8 YANG Cheng-min~(1,2),WANG Hong-zhi~1,SUN Zhen-yuan~3,WEI Jian-hua~(1*) (1.Beijing Institute of Agriculture and Forestry, Beijing 100089 China; 2.College of Agronomy, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia Autonomous Region 010018 China; 3.Laboratory of Biotechnology, CAF, Beijing 100091 China);Co-transformation of bar and P5CS Gene Using Bombardment on Lolium perenne L.[J];Acta Agrestia Sinica;2005-01
9 ZHAO Hua bing,MA Zhi ying,ZHAO Hong (College of Agronomy, Agricultural University of Hebei, Baoding 071001, China);Wheat genetic transformation method:reviews,comparisons and prospects[J];Journal of Agricultural University of Hebei;2002-S1
10 DU Xue-ling~(1),ZHENG Zhen-xia~(2),LIU Ping~(2),YANG Zhong-yi~(2),FU Yi-kun~(1)(1. College of Grassland Science, Gansu Agricutural University, Lanzhou 730070,China;2. College of Life Science, Zhongshan University, Guangzhou 510275,China;3. Department of Biology, Huaibei Coal Industry Teachers College, Huaibei 235000, China);The effect of 2,4-D and 6-BA on callus induction of perennial ryegrass[J];Grassland and Turf;2005-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved